Department of Molecular Biology and Immunology, National Institute of Agrobiological Sciences, Tsukuba, Japan.
Proc Jpn Acad Ser B Phys Biol Sci. 2006 May;82(4):165-73. doi: 10.2183/pjab.82.165.
F1-A(y) mice between RR (aabbCC) and C57BL/6J-A(y) (A(y)aBBCC) have a much darker yellow coat color than do C57BL/6J-A(y) . Quantitative trait locus (QTL) analysis was carried out to identify genes responsible for the darker modification of the yellow coat color (this has been traditionally termed "sable"). A significant sable QTL was identified on chromosome 1 (Dmyaq4, LOD score 15.5 for lightness, and 13.4 for color difference), in a chromosomal position similar to that of Dmyaq2, a sable QTL previously identified in C3H/HeJ. Another significant sable QTL was identified on chromosome 4 (Dmyaq5, LOD score 5.6 for lightness, and 4.3 for color difference) near the tyrosinase-related protein 1 (Tyrp1) locus. The effect of Dmyaq5 was significant only in the presence of the RR allele at Dmyaq4, suggesting that the Dmyaq4 (as well as Dmyaq2) is a novel coat color gene that may act up-stream of Tyrp1 signaling to increase eumelanin production.
F1-A(y) 小鼠（RR[aabbCC]和 C57BL/6J-A(y)[A(y)aBBCC]的杂交种）的皮毛颜色比 C57BL/6J-A(y) 更深黄色。为了鉴定导致黄色皮毛颜色变深的基因，我们进行了数量性状基因座（QTL）分析（这种变深通常被称为“貂色”）。在第 1 号染色体上（Dmyaq4，亮度的对数优势分数为 15.5，色差的对数优势分数为 13.4），我们发现了一个显著的貂色 QTL，其染色体位置与先前在 C3H/HeJ 中鉴定的貂色 QTL Dmyaq2 相似。在 4 号染色体上（Dmyaq5，亮度的对数优势分数为 5.6，色差的对数优势分数为 4.3），我们在接近酪氨酸相关蛋白 1（Tyrp1）基因座的位置发现了另一个显著的貂色 QTL。Dmyaq5 的作用仅在 Dmyaq4 存在 RR 等位基因时显著，这表明 Dmyaq4（和 Dmyaq2）是一个新的皮毛颜色基因，它可能在 Tyrp1 信号的上游作用，增加真黑素的产生。
