Suto J, Wakamatsu K, Yamanaka H, Ito S, Sekikawa K
Department of Immunology, National Institute of Animal Health, Tsukuba, Ibaraki 305-0856, Japan.
Mamm Genome. 2000 Aug;11(8):639-44. doi: 10.1007/s003350010136.
Compared with C57BL/6J-A(y)/a, KK-A(y)/a mice have yellow fur that is markedly darker. Furthermore, there is a considerable variation in the tone of color with a continuous range in F(2) progeny produced from C57BL/6J females and KK-A(y)/a males. The aims of this study are to reveal the phenotypic differences between the two A(y) congenic strains and to elucidate the genetic factors responsible for the sooty yellow pigmentation in the KK background. On the basis of a chemical analysis, the sootiness in KK-A(y)/a was the result of increased eumelanin (PTCA) and decreased pheomelanin (AHP). A statistically significant QTL was identified on Chromosome (Chr) 15, responsible for the AHP content. No significant loci responsible for PTCA were identified. On the other hand, on the basis of an optical analysis for color difference and overall sootiness, significant evidence of linkage was identified on the proximal part of Chr 15, in the region similar to AHP QTL. The overall sootiness is thus controlled solely by the locus on Chr 15 in F(2) progeny; however, the KK allele at this locus significantly increased the AHP content.
与C57BL/6J-A(y)/a相比，KK-A(y)/a小鼠的黄色皮毛颜色明显更深。此外，由C57BL/6J雌性和KK-A(y)/a雄性产生的F(2)后代的毛色深浅存在相当大的差异，呈连续变化。本研究的目的是揭示两种A(y)同源近交系之间的表型差异，并阐明KK背景中导致深黄色素沉着的遗传因素。基于化学分析，KK-A(y)/a中的深色是真黑素（PTCA）增加和褐黑素（AHP）减少的结果。在15号染色体（Chr）上鉴定出一个对AHP含量有显著影响的数量性状基因座（QTL）。未发现对PTCA有显著影响的基因座。另一方面，基于对色差和整体深色的光学分析，在15号染色体近端与AHP QTL相似的区域发现了显著的连锁证据。因此，F(2)后代的整体深色仅由15号染色体上的基因座控制；然而，该基因座上的KK等位基因显著增加了AHP含量。
