低钙饮食对去卵巢小鼠骨骼的影响。
The effect of low calcium diet on bone in ovariectomized mice.
作者信息
Minematsu A, Yoshimura O, Yotsuji H, Ichigo H, Takayanagi K, Kobayashi R, Hosoda M, Sasaki H, Maejima H, Matsuda Y, Tanaka S, Kanemura N, Matsuo A
机构信息
Institute of Health Sciences, Hiroshima University School of Medicine.
Hiroshima Municipal Industrial Technology Center.
出版信息
J Jpn Phys Ther Assoc. 2000;3(1):13-6. doi: 10.1298/jjpta.3.13.
This study investigates the effects of Ca on bone in the ovariectomized mice. Twenty-six female ICR mice aged 5 weeks were used. They were ovariectomized (OVX) or sham-operated (SHAM) and fed standard mouse diet (SF) or special low calcium diet (L.Ca), respectively. All animals were sacrificed at day 100 after operation. Mechanical strength of the left femur and tibia was measured by the three-point bending strength test. The bones were dried, weighed and burned to ash. Correlation between mechanical strength and ash content was found. A specimen of the right tibia was prepared. Mechanical strength, ash content and ratio of dry bone weight to body weight of the femur and tibia in OVX and L.Ca mice were significantly less than in SHAM and SF mice. SHAM/SF mice and OVX/L.Ca group showed highest and lowest values in all cases. The values for the femur and tibia in OVX/SF mice were lower than in SHAM/SF group and in OVX/L.Ca group were less than in OVX/SF mice. Correlation coefficients for mechanical strength and ash content were 0.704 and 0.776 for the femur and tibia. Ca is thus related to inhibition of bone loss and maintenance of bone mass and effective prevention of osteoporosis.
本研究调查了钙对去卵巢小鼠骨骼的影响。使用了26只5周龄的雌性ICR小鼠。它们分别接受了去卵巢手术（OVX）或假手术（SHAM），并分别喂食标准小鼠饲料（SF）或特殊低钙饲料（L.Ca）。所有动物在手术后第100天被处死。通过三点弯曲强度测试测量左股骨和胫骨的机械强度。将骨骼干燥、称重并烧成灰。发现了机械强度与灰分含量之间的相关性。制备了右胫骨的样本。OVX和L.Ca小鼠的股骨和胫骨的机械强度、灰分含量以及干骨重量与体重的比值均显著低于SHAM和SF小鼠。在所有情况下，SHAM/SF小鼠和OVX/L.Ca组分别显示出最高和最低值。OVX/SF小鼠的股骨和胫骨的值低于SHAM/SF组，而OVX/L.Ca组的值低于OVX/SF小鼠。股骨和胫骨的机械强度与灰分含量的相关系数分别为0.704和0.776。因此，钙与抑制骨质流失、维持骨量以及有效预防骨质疏松症有关。
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