School of Kinesiology, Molecular and Applied Sciences Laboratory, Auburn University, 301 Wire Road, Office 286, Auburn, AL 36849 USA.
4Life Research USA, LLC, Sandy, UT USA.
J Int Soc Sports Nutr. 2015 Mar 13;12:14. doi: 10.1186/s12970-015-0076-9. eCollection 2015.
We examined the acute effects of different dietary protein sources (0.19 g, dissolved in 1 ml of water) on skeletal muscle, adipose tissue and hypothalamic satiety-related markers in fasted, male Wistar rats (~250 g).
Oral gavage treatments included: a) whey protein concentrate (WPC, n = 15); b) 70:30 hydrolyzed whey-to-hydrolyzed egg albumin (70 W/30E, n = 15); c) 50 W/50E (n = 15); d) 30 W/70E (n = 15); and e) 1 ml of water with no protein as a fasting control (CTL, n = 14).
Skeletal muscle analyses revealed that compared to CTL: a) phosphorylated (p) markers of mTOR signaling [p-mTOR (Ser2481) and p-rps6 (Ser235/236)] were elevated 2-4-fold in all protein groups 90 min post-treatment (p < 0.05); b) WPC and 70 W/30E increased muscle protein synthesis (MPS) 104% and 74% 180 min post-treatment, respectively (p < 0.05); and c) 70 W/30E increased p-AMPKα (Thr172) 90 and 180-min post-treatment as well as PGC-1α mRNA 90 min post-treatment. Subcutaneous (SQ) and omental fat (OMAT) analyses revealed: a) 70 W/30 W increased SQ fat phosphorylated hormone-sensitive lipase [p-HSL (Ser563)] 3.1-fold versus CTL and a 1.9-4.4-fold change versus all other test proteins 180 min post-treatment (p < 0.05); and b) WPC, 70 W/30E and 50 W/50E increased OMAT p-HSL 3.8-6.5-fold 180 min post-treatment versus CTL (p < 0.05). 70 W/30E and 30 W/70E increased hypothalamic POMC mRNA 90 min post-treatment versus CTL rats suggesting a satiety-related response may have occurred in the former groups. However, there was a compensatory increase in orexigenic AGRP mRNA in the 70 W/30E group 90 min post-treatment versus CTL rats, and there was a compensatory increase in orexigenic NPY mRNA in the 30 W/70E group 90 min post-treatment versus CTL rats.
Higher amounts of whey versus egg protein stimulate the greatest post-treatment anabolic skeletal muscle response, though test proteins with higher amounts of WPH more favorably affected post-treatment markers related to adipose tissue lipolysis.
我们研究了不同膳食蛋白质源（0.19g，溶解于 1ml 水中）对禁食雄性 Wistar 大鼠（~250g）骨骼肌、脂肪组织和下丘脑饱腹感相关标志物的急性影响。
口服灌胃处理包括：a）乳清蛋白浓缩物（WPC，n=15）；b）70:30 水解乳清蛋白至水解卵白蛋白（70W/30E，n=15）；c）50W/50E（n=15）；d）30W/70E（n=15）；e）1ml 不含蛋白质的水作为禁食对照（CTL，n=14）。
骨骼肌分析显示，与 CTL 相比：a）mTOR 信号的磷酸化（p）标志物[p-mTOR（Ser2481）和 p-rps6（Ser235/236）]在所有蛋白质组中 90min 后处理时升高 2-4 倍（p<0.05）；b）WPC 和 70W/30E 分别在 180min 后处理时将肌肉蛋白质合成（MPS）提高了 104%和 74%（p<0.05）；c）70W/30E 在 90 和 180min 后处理时增加了 p-AMPKα（Thr172）和 PGC-1α mRNA 的表达，在 90min 后处理时增加了 p-HSL（Ser563）的表达。皮下（SQ）和网膜脂肪（OMAT）分析显示：a）70W/30W 在 180min 后处理时，与 CTL 相比，SQ 脂肪的磷酸化激素敏感脂肪酶[p-HSL（Ser563）]增加了 3.1 倍，与所有其他测试蛋白相比增加了 1.9-4.4 倍（p<0.05）；b）WPC、70W/30E 和 50W/50E 在 180min 后处理时，与 CTL 相比，OMAT 的 p-HSL 增加了 3.8-6.5 倍（p<0.05）。70W/30E 和 30W/70E 在 90min 后处理时增加了下丘脑 POMC mRNA 的表达，这表明在前两组中可能发生了饱腹感相关反应。然而，70W/30E 组在 90min 后处理时，食欲刺激型 AGRP mRNA 代偿性增加，而 30W/70E 组在 90min 后处理时，食欲刺激型 NPY mRNA 代偿性增加。
与蛋蛋白相比，较高剂量的乳清蛋白刺激了最大的治疗后合成代谢骨骼肌反应，但含有较高 WPH 的测试蛋白更有利于治疗后与脂肪组织脂肪分解相关的标志物。
