Department of Gynecology, Traditional Chinese Medical Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang 830000, China.
Department of Morphology Center, Xinjiang Medical University, Urumqi, Xinjiang 830000, China.
Evid Based Complement Alternat Med. 2015;2015:317586. doi: 10.1155/2015/317586. Epub 2015 Feb 22.
Controlled ovarian hyperstimulation (COH) is widely used in assisted reproductive technology (ART), but it often leads to precocious maturation of the endometrium such that it impairs embryonic implantation and limits pregnancy rates. Previous studies have shown the traditional Chinese medicine, the Zishen Yutai pill (ZYP), to be effective in treatment of threatened as well as recurrent miscarriages, and it can improve embryonic implantation rates in patients undergoing IVF treatment. In the present study, the ZYP has been found to ameliorate precocious endometrial maturation in a mouse model of different COH. Molecular evaluations, real-time PCR, relative RT-PCR, Western blotting, and immunohistochemistry have indicated that the ZYP increased the expression of HOXA10, an important marker of uterine receptivity. Elevation of HOXA10 led to further upregulation of its target gene, integrin β3, and downregulation of EMX2, two additional markers of uterine receptivity. In this way, the ZYP may mitigate COH-induced precocious maturation of the endometrium and improve uterine receptivity by upregulating HOXA10.
控制性卵巢刺激（COH）广泛应用于辅助生殖技术（ART），但它常导致子宫内膜早熟，从而损害胚胎着床并降低妊娠率。既往研究表明，中药滋肾育胎丸（ZYP）对治疗先兆流产和复发性流产有效，可提高接受体外受精治疗患者的胚胎着床率。本研究发现，ZYP 可改善不同 COH 小鼠模型的子宫内膜早熟。分子评估、实时 PCR、相对 RT-PCR、Western 印迹和免疫组织化学表明，ZYP 增加了 HOXA10 的表达，HOXA10 是子宫容受性的重要标志物。HOXA10 的升高进一步上调了其靶基因整合素 β3，而下调了另两个子宫容受性标志物 EMX2。因此，ZYP 可能通过上调 HOXA10 来减轻 COH 引起的子宫内膜早熟并改善子宫容受性。
