Da Yunmeng, Niu Kaiyu, Wang Ke, Cui Guangxia, Wang Wenjuan, Jin Biao, Sun Yu, Jia Jing, Qin Lihua, Bai Wenpei
Department of Stomatology, General Hospital of Chinese People's Armed Police Forces, Beijing, China.
Department of Anatomy and Embryology, Peking University Health Science Center, Beijing, China.
PLoS One. 2015 Mar 20;10(3):e0121470. doi: 10.1371/journal.pone.0121470. eCollection 2015.
This study aims to observe the effects of estradiol and Cimicifuga racemosa on the lacrimal gland and submandibular gland of ovariectomized rats. We randomly divided 20 adult female SD rats into four groups-a sham-operated group (SHAM), ovariectomized (OVX) group, ovariectomized group treated with estradiol (OVX+ E), and ovariectomized group treated with the isopropanolic extract of Cimicifuga racemosa (OVX+ iCR). The SHAM group and OVX group used distilled water to instead the drugs. Two weeks after ovariectomy, the estradiol and iCR were administered for 4 weeks. Next, we used H&E staining and electron microscopy to observe any histological changes in the lacrimal and submandibular glands and immunohistochemical staining to observe the expressions of cleaved caspase-3 (Casp-3) and Cu-Zn SOD (superoxide dismutase). The H&E staining find that both drugs can prevent the cells of area from shrinkage in the two kinds of gland. But under the electron microscopy, estradiol and iCR have different efficacy. Estradiol is more effective at protecting mitochondria in lacrimal gland acinar cells than iCR, and iCR is more effective at suppressing endoplasmic reticulum expansion than estradiol. Both estradiol and iCR have a similar protective function on mitochondria in the submandibular gland. The protective function of the two glands may inhibit apoptosis by suppressing the expression of Casp-3. In addition, iCR increases the expression of Cu-Zn SOD in duct system of submandibular gland. The results suggest that both estradiol and iCR confer a protective effect on the lacrimal and submandibular glands of ovariectomized rats via different mechanisms.
本研究旨在观察雌二醇和总状升麻对去卵巢大鼠泪腺和下颌下腺的影响。我们将20只成年雌性SD大鼠随机分为四组——假手术组（SHAM）、去卵巢组（OVX）、去卵巢后用雌二醇治疗组（OVX+E）和去卵巢后用总状升麻异丙醇提取物治疗组（OVX+iCR）。SHAM组和OVX组用蒸馏水替代药物。去卵巢两周后，给予雌二醇和iCR治疗4周。接下来，我们用苏木精-伊红染色和电子显微镜观察泪腺和下颌下腺的组织学变化，并用免疫组织化学染色观察裂解的半胱天冬酶-3（Casp-3）和铜锌超氧化物歧化酶（Cu-Zn SOD）的表达。苏木精-伊红染色发现两种药物均可防止两种腺体中该区域的细胞萎缩。但在电子显微镜下，雌二醇和iCR有不同的效果。雌二醇在保护泪腺腺泡细胞中的线粒体方面比iCR更有效，而iCR在抑制内质网扩张方面比雌二醇更有效。雌二醇和iCR在下颌下腺中对线粒体具有相似的保护作用。两种腺体的保护作用可能通过抑制Casp-3的表达来抑制细胞凋亡。此外，iCR增加了下颌下腺导管系统中Cu-Zn SOD的表达。结果表明，雌二醇和iCR均通过不同机制对去卵巢大鼠的泪腺和下颌下腺具有保护作用。
