Talwar G P, Gupta S K, Singh V, Sahal D, Iyer K S, Singh O
Proc Natl Acad Sci U S A. 1985 Feb;82(4):1228-31. doi: 10.1073/pnas.82.4.1228.
A monoclonal antibody generated against the decapeptide gonadotropin-releasing hormone (GnRH) was effective in intercepting the bioactivity of the hormone; it blocked ovulation in rats. The antibody reacted optimally with the native hormone. Substitution of amide at the COOH terminus by a carboxyl group decreased immunoreactivity by a factor of 200. The antibody recognized the amino acid sequences 4-6, 7-10, and 4-10 to a variable degree, which suggests that the epitope has a conformation involving the entire molecule, with the NH2- and COOH-terminal regions probably in proximity. The antibody was also competent to suppress the progression of estrus in dogs, an indication that GnRH may play an inductive role in the reproductive function of dogs.
一种针对十肽促性腺激素释放激素（GnRH）产生的单克隆抗体能够有效拦截该激素的生物活性；它能阻断大鼠排卵。该抗体与天然激素反应最佳。将COOH末端的酰胺替换为羧基会使免疫反应性降低200倍。该抗体对氨基酸序列4 - 6、7 - 10和4 - 10有不同程度的识别，这表明表位具有涉及整个分子的构象，NH2 - 和COOH - 末端区域可能相邻。该抗体也能够抑制犬发情的进展，这表明GnRH可能在犬的生殖功能中起诱导作用。
