Gupta S K, Singh O, Talwar G P
Am J Reprod Immunol Microbiol. 1985 Mar;7(3):104-8. doi: 10.1111/j.1600-0897.1985.tb00266.x.
Mouse hybrid cell clones secreting antigonadotropin releasing hormone monoclonal antibody were developed by fusion of SP2/O-Ag 1.4 myeloma cells with splenocytes of mouse immunized with gonadotropin releasing hormone (GnRH) tagged to tetanus toxoid. The product of hybrid cell clones obtained as ascites fluid from mouse peritoneal cavity had a titre of 10(6) (30-40% binding of 125I-GnRH) in radioimmunoassay. The antibody was IgG2a and Kappa. The association constant (Ka) of the product of hybrid cell clone P(8)16(62) for binding with GnRH was 1.2 X 10(9) L/mole. The monoclonal antibody (P(8)16(62)) was highly specific for the native GnRH and devoid of reactivity with thyroid releasing hormone as tested in competitive radioimmunoassay. The recognition for GnRH agonists by monoclonal was 387-fold less with D-Ser (But)6 des Gly10 GnRH ethylamide and 608-fold less with Bz1-His6 GnRH. Monoclonal anti-GnRH antibody was competent to neutralize the in vivo bioactivity of the hormone as evident by the block of estrus cycle and termination of pregnancy in mice. Termination of pregnancy in animals receiving anti-GnRH monoclonal could be prevented by administration of progesterone.
通过将SP2/O-Ag 1.4骨髓瘤细胞与用破伤风类毒素标记的促性腺激素释放激素（GnRH）免疫的小鼠脾细胞融合，获得了分泌抗促性腺激素释放激素单克隆抗体的小鼠杂交细胞克隆。从小鼠腹腔获得的腹水液中杂交细胞克隆产物在放射免疫测定中的效价为10(6)（125I-GnRH结合率为30-40%）。该抗体为IgG2a和κ链。杂交细胞克隆P(8)16(62)产物与GnRH结合的缔合常数（Ka）为1.2×10(9) L/摩尔。在竞争性放射免疫测定中测试，单克隆抗体（P(8)16(62)）对天然GnRH具有高度特异性，与促甲状腺激素释放激素无反应性。单克隆抗体对GnRH激动剂的识别能力，与D-Ser(But)6 des Gly10 GnRH乙酰胺相比低387倍，与Bz1-His6 GnRH相比低608倍。单克隆抗GnRH抗体能够中和该激素的体内生物活性，这在小鼠发情周期阻断和妊娠终止中得到了证明。接受抗GnRH单克隆抗体的动物妊娠终止可通过给予孕酮来预防。
