Rossetti Fernanda F, Schneck Emanuel, Fragneto Giovanna, Konovalov Oleg V, Tanaka Motomu
†Physical Chemistry of Biosystems, Physical Chemistry Institute, University of Heidelberg, D-69120 Heidelberg, Germany.
‡Institute for Integrated Cell-Material Sciences (WPI iCeMS), Kyoto University, 606-8501 Kyoto, Japan.
Langmuir. 2015 Apr 21;31(15):4473-80. doi: 10.1021/la504253p. Epub 2015 Apr 7.
To understand the generic role of soft, hydrated biopolymers in adjusting interfacial interactions at biological interfaces, we designed a defined model of the cell-extracellular matrix contacts based on planar lipid membranes deposited on polymer supports (polymer-supported membranes). Highly uniform polymer supports made out of regenerated cellulose allow for the control of film thickness without changing the surface roughness and without osmotic dehydration. The complementary combination of specular neutron reflectivity and high-energy specular X-ray reflectivity yields the equilibrium membrane-substrate distances, which can quantitatively be modeled by computing the interplay of van der Waals interaction, hydration repulsion, and repulsion caused by the thermal undulation of membranes. The obtained results help to understand the role of a biopolymer in the interfacial interactions of cell membranes from a physical point of view and also open a large potential to generally bridge soft, biological matter and hard inorganic materials.
为了理解柔软的水合生物聚合物在调节生物界面处界面相互作用中的一般作用，我们基于沉积在聚合物载体（聚合物支撑膜）上的平面脂质膜设计了一种确定的细胞 - 细胞外基质接触模型。由再生纤维素制成的高度均匀的聚合物载体能够在不改变表面粗糙度且不发生渗透脱水的情况下控制膜厚度。镜面中子反射率和高能镜面X射线反射率的互补结合产生了平衡的膜 - 底物距离，这可以通过计算范德华相互作用、水合排斥以及由膜的热波动引起的排斥之间的相互作用进行定量建模。所获得的结果有助于从物理角度理解生物聚合物在细胞膜界面相互作用中的作用，并且还为普遍连接柔软的生物物质和坚硬的无机材料开辟了巨大潜力。
