Lertwanawatana Proyphon, Frazier Richard A, Niranjan Keshavan
Department of Food and Nutritional Sciences, University of Reading, Whiteknights, PO Box 226, Reading RG6 6AP, United Kingdom.
Food Chem. 2015 Aug 15;181:85-93. doi: 10.1016/j.foodchem.2015.02.005. Epub 2015 Feb 21.
Cassava starch, typically, has resistant starch type 3 (RS3) content of 2.4%. This paper shows that the RS3 yields can be substantially enhanced by debranching cassava starch using pullulanase followed by high pressure or cyclic high-pressure annealing. RS3 yield of 41.3% was obtained when annealing was carried out at 400MPa/60°C for 15 min, whereas it took nearly 8h to obtain the same yield under conventional atmospheric annealing at 60°C. The yield of RS3 could be further significantly increased by annealing under 400 MPa/60°C pressure for 15 min followed by resting at atmospheric pressure for 3h 45 min, and repeating this cycle for up to six times. Microstructural surface analysis of the product under a scanning electron microscope showed an increasingly rigid density of the crystalline structure formed, confirming higher RS3 content.
木薯淀粉通常含有2.4%的3型抗性淀粉（RS3）。本文表明，使用普鲁兰酶对木薯淀粉进行脱支，然后进行高压或循环高压退火，可以大幅提高RS3的产量。在400MPa/60°C下退火15分钟，RS3产量可达41.3%，而在传统的60°C常压退火条件下，获得相同产量则需要近8小时。在400MPa/60°C压力下退火15分钟，然后在常压下静置3小时45分钟，并重复此循环多达六次，RS3的产量可进一步显著提高。在扫描电子显微镜下对产物进行微观结构表面分析，结果显示形成的晶体结构密度越来越高，证实了RS3含量更高。
