King Mongkut's University of Technology Thonburi, Bangkuntien, Bangkok, Thailand.
J Biosci Bioeng. 2010 Jan;109(1):9-14. doi: 10.1016/j.jbiosc.2009.06.021. Epub 2009 Jul 21.
The objective of this work was to remove linamarin in starch from cassava (Manihot esculenta Crantz cv. KU-50) roots, a high-cyanogen variety by using plant cell wall-degrading enzymes, xylanase and cellulase. The combination of xylanase from Bacillus firmus K-1 and xylanase and cellulase from Paenibacillus curdlanolyticus B-6 at the ratio of 1:9 showed the maximum synergism at 1.8 times for hydrolyzing cassava cortex cell walls and releasing linamarase. Combined enzyme treatment enhanced linamarin liberation from the parenchyma by 90%. In addition, when the combined enzymes were applied for detoxification during cassava starch production, a low-cyanide-product was obtained with decreased linamarin concentration (96%) compared to non-enzyme treated tissues. Based on these results, xylanase and cellulase treatment is a good method for low-cyanide-cassava starch production and could be applied for detoxification of cassava products during processing.
本工作旨在通过使用植物细胞壁降解酶木聚糖酶和纤维素酶从木薯（Manihot esculenta Crantz cv. KU-50）根中去除氰化物含量高的亚麻氰苷，木薯品种 KU-50 中的氰化物含量高。Bacillus firmus K-1 的木聚糖酶与 Paenibacillus curdlanolyticus B-6 的木聚糖酶和纤维素酶以 1:9 的比例组合使用时，对水解木薯皮层细胞壁和释放亚麻苦苷酶的协同作用最大，可达 1.8 倍。复合酶处理可使胚乳中的亚麻苦苷释放提高 90%。此外，在木薯淀粉生产过程中应用复合酶进行解毒时，与未经酶处理的组织相比，氰化物产物的浓度降低（96%）。基于这些结果，木聚糖酶和纤维素酶处理是一种生产低氰化物木薯淀粉的好方法，可应用于加工过程中木薯制品的解毒。
