Institute of Environmental Engineering, National Chiao Tung University, Hsinchu 30010, Taiwan.
Department of Bioenvironmental Engineering, Chung-Yuan Christian University, 200 Chung Pei Road, Chung-Li 32023, Taiwan.
Bioresour Technol. 2015 Aug;190:480-6. doi: 10.1016/j.biortech.2015.02.064. Epub 2015 Feb 20.
Simultaneous partial nitrification, anammox and denitrification (SNAD) process was started-up in a 2.5L sequencing batch biofilm reactor (SBBR) using novel biomass carriers. The SNAD process took only 51d for start-up at nitrogen loading rate (NLR) and organic loading rate (OLR) of 120 and 60g/m(3)-d, respectively. Long-term stable operation of SNAD process was observed at NLR and OLR of 360 and 180g/m(3)-d with average total nitrogen and COD removal efficiencies of >88% and >90%, respectively. The values of conversion ratio [Formula: see text] remained below 0.11 after the start-up period, which further confirmed the long-term stability of SNAD process. Results of polymerase chain reaction (PCR), qualitative PCR, and scanning electron microscopic (SEM) analysis of sludge samples confirmed the co-existence and enrichment of AOB, anammox bacteria and denitrifying bacteria in the reactor and biofilm formation on to the carriers.
采用新型生物载体，在 2.5L 序批式生物膜反应器（SBBR）中启动同时硝化-反硝化-厌氧氨氧化（SNAD）工艺。在氮负荷（NLR）和有机负荷（OLR）分别为 120 和 60g/m³·d 的条件下，SNAD 工艺仅用 51d 即可启动。在 NLR 和 OLR 分别为 360 和 180g/m³·d 的条件下，SNAD 工艺能够长期稳定运行，总氮和 COD 的平均去除效率分别大于 88%和 90%。启动后[Formula: see text]值一直低于 0.11，进一步证实了 SNAD 工艺的长期稳定性。聚合酶链反应（PCR）、定性 PCR 和污泥样品扫描电子显微镜（SEM）分析的结果证实了反应器和载体上 AOB、厌氧氨氧化菌和反硝化菌的共存和富集以及生物膜的形成。
