Zheng Zhaoming, Li Jun, Ma Jing, Du Jia, Bian Wei, Li Yun, Zhang Yanzhuo, Zhao Baihang
The College of Architecture and Civil Engineering, Beijing University of Technology, Beijing, 100124, China.
Biodegradation. 2016 Nov;27(4-6):195-208. doi: 10.1007/s10532-016-9766-5. Epub 2016 Jun 21.
The simultaneous partial nitrification, anammox and denitrification (SNAD) process for treating domestic wastewater was investigated in a sequencing batch reactor (SBR). The SBR was operated with air flow rate of 500 L h at 30 °C. Domestic wastewater was used as influent and Kaldnes rings were used as biomass carriers. In the beginning, long aeration condition was implemented to cultivate nitrification biofilm. Afterwards, intermittent aerobic condition was conducted during the cycle operation. The influent organic matter loading rate was improved by reducing the aeration and mixing times. Consequently, when the SNAD biofilm reactor was fed with the organic matter loading rate of 0.77 (kg COD m d), the bio-bubbles appeared in the reactor and the total inorganic nitrogen (TIN) removal efficiency decreased. After the organic matter loading rate decreased to 0.67 (kg COD m d), the reactor showed excellent nitrogen removal performance. The TIN removal efficiency varied between 80 and 90 %, and the average TIN removal loading rate was 0.22 (kg TIN m d). Additionally, the scanning electron microscope (SEM) observation confirmed that the anammox bacteria located in the inner part of the carriers. Finally, the microbial community analysis of 16S rRNA gene cloning revealed that the anammox bacteria on the carriers consisted of three main genuses: Candidatus Brocadia sp., Candidatus Brocadia caroliniensis and Candidatus Brocadia fulgida.
在序批式反应器（SBR）中研究了用于处理生活污水的同步短程硝化、厌氧氨氧化和反硝化（SNAD）工艺。SBR在30℃下以500 L/h的空气流量运行。以生活污水作为进水，以Kaldnes环作为生物量载体。开始时，采用长时间曝气条件培养硝化生物膜。之后，在循环运行期间采用间歇好氧条件。通过减少曝气和混合时间提高进水有机物负荷率。结果，当SNAD生物膜反应器的进水有机物负荷率为0.77（kg COD/m³·d）时，反应器中出现生物气泡，总无机氮（TIN）去除效率下降。当有机物负荷率降至0.67（kg COD/m³·d）后，反应器表现出优异的脱氮性能。TIN去除效率在80%至90%之间变化，平均TIN去除负荷率为0.22（kg TIN/m³·d）。此外，扫描电子显微镜（SEM）观察证实厌氧氨氧化菌位于载体内部。最后，通过16S rRNA基因克隆进行的微生物群落分析表明，载体上的厌氧氨氧化菌由三个主要属组成：Candidatus Brocadia sp.、Candidatus Brocadia caroliniensis和Candidatus Brocadia fulgida。
