Agaoglu Ozgecan Korkmaz, Agaoglu Ali Reha, Guzeloglu Aydin, Kurar Ercan, Kayis Seyit Ali, Ozmen Ozlem, Schäfer-Somi Sabine, Aslan Selim
Department of Animal Science, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, Turkey.
Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, Turkey.
Theriogenology. 2015 Jul 1;84(1):24-33. doi: 10.1016/j.theriogenology.2015.02.009. Epub 2015 Feb 16.
Hypoxia-inducible factors (HIFs) and vascular endothelial growth factor (VEGF) have critical roles during the development of the fetomaternal unit. The HIFs regulate placentation and vascularization by stimulation of VEGF gene expression. This study aimed to investigate the expression profiles of HIF gene family and VEGF in the cat uterus during pregnancy. Tissue samples of the whole uterine wall were collected after ovariohysterectomy and allocated to the following groups: embryo positive (group 1 [G1], n = 7, 7 days after mating), early pregnancy (group 2 [G2], n = 7, 20 days after mating), mid-pregnancy (group 3 [G3], n = 7, 24 days after mating), late pregnancy (group 4 [G4], n = 7, 30-45 days after mating), and oocyte positive groups (group 5 [G5], n = 7, 7 days after induction of ovulation with GnRH analog). Relative mRNA levels were determined by real-time polymerase chain reaction. As housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase was used. The relative gene expression of HIF1A in G5 was found to be significantly higher than that of other groups (G1, G2, G3, and G4) (P < 0.05). In addition, the expression of HIF2A in G5 was higher than that of G1 and HIF2A gene expression at placentation sites of G4 was higher than in G1, G2, and G3 (P < 0.05). Immunohistochemistry indicated that HIF1A, HIF2A, and VEGF expressions were observed in different cell types of uterine and placental tissues in late pregnancy and oocyte groups. The expression of HIF3A did not change significantly in any group investigated. These observations suggest that HIFs and VEGF may play a role in the establishment and development of pregnancy.
缺氧诱导因子（HIFs）和血管内皮生长因子（VEGF）在母胎单位的发育过程中发挥着关键作用。HIFs通过刺激VEGF基因表达来调节胎盘形成和血管生成。本研究旨在调查猫孕期子宫中HIF基因家族和VEGF的表达谱。卵巢子宫切除术后收集整个子宫壁的组织样本，并分为以下几组：胚胎阳性组（第1组[G1]，n = 7，交配后7天）、早孕组（第2组[G2]，n = 7，交配后20天）、中孕组（第3组[G3]，n = 7，交配后24天）、晚孕组（第4组[G4]，n = 7，交配后30 - 45天）和卵母细胞阳性组（第5组[G5]，n = 7，用GnRH类似物诱导排卵后7天）。通过实时聚合酶链反应测定相对mRNA水平。使用甘油醛 - 3 - 磷酸脱氢酶作为看家基因。发现G5中HIF1A的相对基因表达显著高于其他组（G1、G2、G3和G4）（P < 0.05）。此外，G5中HIF2A的表达高于G1，G4胎盘部位的HIF2A基因表达高于G1、G2和G3（P < 0.05）。免疫组织化学表明，在晚孕组和卵母细胞组的子宫和胎盘组织的不同细胞类型中观察到HIF1A、HIF2A和VEGF的表达。在所研究的任何组中，HIF3A的表达均无显著变化。这些观察结果表明，HIFs和VEGF可能在妊娠的建立和发育中发挥作用。
