基于模型的细胞培养种子批布局策略在实验室规模得到验证。
Model-based strategy for cell culture seed train layout verified at lab scale.
作者信息
Kern Simon, Platas-Barradas Oscar, Pörtner Ralf, Frahm Björn
机构信息
Biotechnology and Bioprocess Engineering, Faculty of Life Science Technologies, Ostwestfalen-Lippe University of Applied Sciences, Liebigstr. 87, 32657, Lemgo, Germany.
Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, Denickestr. 15, 21073, Hamburg, Germany.
出版信息
Cytotechnology. 2016 Aug;68(4):1019-32. doi: 10.1007/s10616-015-9858-9. Epub 2015 Mar 21.
Cell culture seed trains-the generation of a sufficient viable cell number for the inoculation of the production scale bioreactor, starting from incubator scale-are time- and cost-intensive. Accordingly, a seed train offers potential for optimization regarding its layout and the corresponding proceedings. A tool has been developed to determine the optimal points in time for cell passaging from one scale into the next and it has been applied to two different cell lines at lab scale, AGE1.HN AAT and CHO-K1. For evaluation, experimental seed train realization has been evaluated in comparison to its layout. In case of the AGE1.HN AAT cell line, the results have also been compared to the formerly manually designed seed train. The tool provides the same seed train layout based on the data of only two batches.
细胞培养种子扩增流程——从培养箱规模开始，为生产规模生物反应器接种生成足够数量的活细胞——既耗时又成本高昂。因此，种子扩增流程在其布局和相应操作方面具有优化潜力。已开发出一种工具来确定细胞从一个规模传代到下一个规模的最佳时间点，并已在实验室规模应用于两种不同的细胞系，AGE1.HN AAT和CHO-K1。为了进行评估，已将实验性种子扩增流程的实现与其布局进行了比较。对于AGE1.HN AAT细胞系，还将结果与之前手动设计的种子扩增流程进行了比较。该工具仅基于两批数据就能提供相同的种子扩增流程布局。
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