Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
Department of Endocrinology, 9th People's Hospital of Chongqing, Chongqing 400700, People's Republic of China.
Biosens Bioelectron. 2015 Aug 15;70:89-97. doi: 10.1016/j.bios.2015.03.021. Epub 2015 Mar 11.
A novel signal-on electrochemiluminescence (ECL) biosensor for detecting concanavalin A (Con A) was fabricated with phenoxy dextran-graphite-like carbon nitride (DexP-g-C3N4) as signal probe. In this construction strategy, the nanocomposites of three-dimensional graphene and gold nanoparticles (3D-GR-AuNPs) were used as matrix for high loading of glucose oxidase (GOx), which served as recognition element for bounding Con A. Con A further interacted with DexP-g-C3N4 through a specific carbohydrate-Con A interaction to achieve a sandwiched scheme. With the increase of Con A incubated onto the electrode, the ECL signal resulted from DexP-g-C3N4 would enhance, thus achieving a signal-on ECL biosensor for Con A detection. Due to the integration of the virtues of 3D-GR-AuNPs and the excellent ECL performance of DexP-g-C3N4, the prepared biosensor exhibits a wide linear response range from 0.05 ng/mL to 100 ng/mL and a low detection limit of 17 pg/mL (S/N=3).
一种用于检测刀豆球蛋白 A（Con A）的新型信号开启电化学发光（ECL）生物传感器，采用苯氧基葡聚糖-类石墨相氮化碳（DexP-g-C3N4）作为信号探针。在这种构建策略中，三维石墨烯和金纳米粒子（3D-GR-AuNPs）的纳米复合材料被用作高负载葡萄糖氧化酶（GOx）的基质，GOx 作为识别元件与 Con A 结合。Con A 进一步通过特定的碳水化合物- Con A 相互作用与 DexP-g-C3N4 相互作用，以实现三明治方案。随着孵育到电极上的 Con A 的增加，来自 DexP-g-C3N4 的 ECL 信号将增强，从而实现用于 Con A 检测的信号开启 ECL 生物传感器。由于 3D-GR-AuNPs 的优点和 DexP-g-C3N4 的优异 ECL 性能的集成，所制备的生物传感器表现出从 0.05ng/mL 到 100ng/mL 的宽线性响应范围和 17pg/mL（S/N=3）的低检测限。
