School of Pharmacy, Jiangsu University, Zhenjiang, 212013, China.
School of the Environment, Jiangsu University, Zhenjiang, 212013, China.
Mikrochim Acta. 2018 Sep 17;185(10):463. doi: 10.1007/s00604-018-2997-x.
An electrochemiluminescence (ECL) based assay is described for the determination of the endocrine disruptor bisphenol A (BPA). The method is based on the use of carboxylated graphitic carbon nitride (C-g-CN) carrying an immobilized aptamer against BPA. In the presence of BPA, the ECL signal decreases due to ECL energy transfer from excited-state C-g-CN to the BPA oxidation product. Under the optimal conditions, ECL intensity increases linearly in the 0.1 pM to 1 nM BPA concentration range. The detection limit is as low as 30 fM. The assay has excellent sensitivity, outstanding stability and high selectivity. It was applied to the determination of BPA in spiked water samples. Graphical abstract Aptamer modified carboxylated graphitic carbon nitride was synthesized and applied in an electrochemiluminescence-based aptasensor for bisphenol A.
一种基于电致化学发光（ECL）的测定方法，用于测定内分泌干扰物双酚 A（BPA）。该方法基于使用带有固定化针对 BPA 的适体的羧基化石墨相氮化碳（C-g-CN）。在存在 BPA 的情况下，由于从激发态 C-g-CN 到 BPA 氧化产物的 ECL 能量转移，ECL 信号会降低。在最佳条件下，ECL 强度在 0.1 pM 至 1 nM BPA 浓度范围内呈线性增加。检测限低至 30 fM。该测定法具有出色的灵敏度、卓越的稳定性和高度的选择性。它被应用于测定加标水样中的 BPA。
