Saito M, Yu R K, Cheung N K
Biochem Biophys Res Commun. 1985 Feb 28;127(1):1-7. doi: 10.1016/s0006-291x(85)80117-0.
Four monoclonal antibodies (3F8, 3A7, 3G6, and 2F7) against human neuroblastoma cells have been suggested to react with surface glycolipids of these cells. In this report these monoclonal antibodies were shown to be specific to the disialoganglioside GD2 using a thin-layer chromatography (TLC)-immunostaining method. When mixed human brain gangliosides were developed by TLC in two different solvent systems and incubated with each of the monoclonal antibodies, only GD2 was stained. These antibodies also reacted with highly purified GD2 on the plate. These findings suggest that GD2 provides an antigenic site on the surface of human neuroblastoma cells.
四种针对人神经母细胞瘤细胞的单克隆抗体（3F8、3A7、3G6和2F7）已被证实可与这些细胞的表面糖脂发生反应。在本报告中，通过薄层色谱（TLC）-免疫染色法显示，这些单克隆抗体对双唾液酸神经节苷脂GD2具有特异性。当混合的人脑神经节苷脂在两种不同的溶剂系统中通过TLC展开并与每种单克隆抗体孵育时，只有GD2被染色。这些抗体也与平板上高度纯化的GD2发生反应。这些发现表明，GD2在人神经母细胞瘤细胞表面提供了一个抗原位点。
