Coquil J F, Brunelle G, Guédon J
Biochem Biophys Res Commun. 1985 Feb 28;127(1):226-31. doi: 10.1016/s0006-291x(85)80148-0.
A new type of cGMP binding protein, the activity of which is characteristically stimulated by methylisobutylxanthine, has been previously discovered in rat lung and platelets (Hamet, P. and Coquil, J.F. (1978) J. Cyclic Nucleotide Res. 4, 281-290). In the present study, we demonstrate the occurrence of this protein in soluble extracts of a variety of rat tissues fractionated by a DEAE-Sepharose chromatography. In several tissues (spleen, lung and brain) the binding activity of this protein was of the same order of magnitude as that of the cGMP-dependent protein kinase.
一种新型的cGMP结合蛋白，其活性特征性地受到甲基异丁基黄嘌呤的刺激，先前已在大鼠肺和血小板中发现（哈梅特，P.和科基尔，J.F.（1978年）《环核苷酸研究杂志》4，281 - 290）。在本研究中，我们证明了这种蛋白存在于通过DEAE - 琼脂糖色谱法分级分离的多种大鼠组织的可溶性提取物中。在几种组织（脾脏、肺和脑）中，这种蛋白的结合活性与cGMP依赖性蛋白激酶的结合活性处于同一数量级。
