Scipioni Angela, Stefanini Stefania, Santone Rocco, Giorgi Mauro
Department of Cellular and Developmental Biology, University La Sapienza, Piazzale Aldo Moro 5, Rome, 00185, Italy.
Histochem Cell Biol. 2005 Nov;124(5):401-7. doi: 10.1007/s00418-005-0057-1. Epub 2005 Nov 3.
Expression of phosphodiesterase 5 (PDE5) in the rat testis at several pre and postnatal developmental stages was investigated by immunohistochemistry. The enzyme was localised in vascular smooth muscle cells, as well as in Leydig and peritubular cells. The latter were identified as myoid, based on their immunoreactivity to desmin and alpha-smooth muscle actin. The presence of PDE5 in myoid cells was confirmed by Western blot analysis and immunohistochemistry performed on highly purified cell fractions, obtained from 16-day-old rats. The expression of PDE5 in these somatic cells of rat testis is discussed in view of the roles played by cGMP signal transduction pathways in the mammalian male reproductive function.
采用免疫组织化学方法研究了磷酸二酯酶5（PDE5）在大鼠出生前后几个发育阶段睾丸中的表达情况。该酶定位于血管平滑肌细胞以及睾丸间质细胞和睾丸周细胞。根据其对结蛋白和α-平滑肌肌动蛋白的免疫反应性，后者被鉴定为类肌细胞。通过蛋白质免疫印迹分析以及对从16日龄大鼠获得的高度纯化细胞组分进行免疫组织化学，证实了类肌细胞中存在PDE5。鉴于环磷酸鸟苷（cGMP）信号转导通路在哺乳动物雄性生殖功能中所起的作用，对大鼠睾丸这些体细胞中PDE5的表达进行了讨论。
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