基于硫化铜纳米颗粒阳离子交换和滚环扩增的DNA/微小RNA化学发光检测
Chemiluminescence detection of DNA/microRNA based on cation-exchange of CuS nanoparticles and rolling circle amplification.
作者信息
Zhang Xiaoru, Liu Hongxia, Li Ruijuan, Zhang Ningbo, Xiong Ying, Niu Shuyan
机构信息
Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P. R. China.
出版信息
Chem Commun (Camb). 2015 Apr 25;51(32):6952-5. doi: 10.1039/c5cc01317h. Epub 2015 Mar 23.
PMID:25797586
Abstract
Cation-exchange-based chemiluminescence amplification (CXCLAmp) was developed in this study. After amplification by rolling circle amplification (RCA), the proposed RCA-CXCLAmp strategy was used to detect miRNA sensitively.
摘要
本研究开发了基于阳离子交换的化学发光放大技术（CXCLAmp）。通过滚环扩增（RCA）进行放大后，所提出的RCA-CXCLAmp策略被用于灵敏地检测微小RNA（miRNA）。
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