Francis Nathan C, Kassam Imara, Nowroozi Bryan, Grundfest Warren S, Taylor Zach D
Department of Bioengineering, University of California Los Angeles, 420 Westwood Plaza, Los Angeles, CA 90024, USA.
Department of Bioengineering, University of California Los Angeles, 420 Westwood Plaza, Los Angeles, CA 90024, USA ; Department of Electrical Engineering, University of California Los Angeles, 420 Westwood Plaza, Los Angeles, CA 90024, USA ; Department of Surgery, University of California Los Angeles, 420 Westwood Plaza, Los Angeles, CA 90024, USA.
Biomed Opt Express. 2015 Feb 17;6(3):827-37. doi: 10.1364/BOE.6.000827. eCollection 2015 Mar 1.
Bacteria biofilms in chronically infected wounds significantly increase the burden of healthcare costs and resources for patients and clinics. Because biofilms are such an effective barrier to standard antibiotic treatment, new methods of therapy need to be developed to combat these infections. Our group has demonstrated the potential of using Laser Generated Shockwaves as a potential therapy to mechanically disrupt the bacterial biofilms covering the wound. Previous studies have used rigid silica glass as the shockwave propagation medium, which is not compatible with the intended clinical application. This paper describes the exploration of five candidate flexible plastic films to replace the glass substrate. Each material measured 0.254 mm thick and was used to generate shockwaves of varying intensities. Shockwave characterization was performed using a high-speed Michelson displacement interferometer and peak stress values obtained in the flexible substrates were compared to glass using one-way nested Analysis of Variance and Tukey HSD post-hoc analysis. Results demonstrate statistically significant differences between substrate material and indicate that polycarbonate achieves the highest peak stress for a given laser fluence suggesting that it is optimal for clinical applications.
慢性感染伤口中的细菌生物膜显著增加了患者和诊所的医疗成本及资源负担。由于生物膜是标准抗生素治疗的有效屏障，因此需要开发新的治疗方法来对抗这些感染。我们团队已证明，利用激光产生的冲击波作为一种潜在疗法，可机械破坏覆盖伤口的细菌生物膜。先前的研究使用刚性石英玻璃作为冲击波传播介质，这与预期的临床应用不兼容。本文描述了对五种候选柔性塑料薄膜的探索，以取代玻璃基板。每种材料的厚度均为0.254毫米，并用于产生不同强度的冲击波。使用高速迈克尔逊位移干涉仪对冲击波进行表征，并使用单向嵌套方差分析和Tukey HSD事后分析，将在柔性基板中获得的峰值应力值与玻璃进行比较。结果表明，基板材料之间存在统计学上的显著差异，并且表明聚碳酸酯在给定激光能量密度下可实现最高峰值应力，这表明它最适合临床应用。
