Aydin Mesut, Yildiz Abdulkadir, Ibiloglu Ibrahim, Ekinci Aysun, Ulger Burak Veli, Yuksel Murat, Bilik Mehmet Zihni, Ozaydogdu Necdet, Ekinci Cenap, Yazgan Umit Can
a Department of Cardiology .
Toxicol Mech Methods. 2015;25(4):296-301. doi: 10.3109/15376516.2015.1025349.
Deltamethrin (DLM) is an insecticide commonly used to protect agricultural crops against pests. QT prolongation with malignant ventricular arrhythmias are amongst the most common cardiovascular complications. DLM intoxication cause decreased level of antioxidant enzymes. Glutamine is the precursor of glutathione which is an antioxidant and has been demonstrated to improve outcome after several critical illnesses.
We hypothesized that glutamine, by means of antioxidant characteristics, may antagonize the cardiotoxic effects of DLM.
All experiments were performed on 8-week-old male Wistar albino rats. The rats were divided into following groups (n = 10); Group I: control, Group II: l-glutamine, Group III: DLM, Group IV: DLM and after 4 h l-glutamine. Total antioxidant status (TAS), total oxidant status (TOS) and parameter analyses were performed in cardiac tissue.
We found that TAS was higher and TOS lower in DLM group. We also found that interstitial edema and inflammatory cell infiltration was significantly more frequent in DLM group and QT and QTc of DLM group were higher than others.
Recent studies have shown that several special amino acids, such as glutamine, glycine, arginine and taurine, exhibit cytoprotective effect on the cardiocyte, and have established the cardioprotective properties of glutamine.
In this study, we showed the protective role of glutamine against cardiotoxic effects of DLM in rats. This protective effect was confirmed by showing both tissue level improvement in oxidative stress markers and improvement in prolonged QT interval.
溴氰菊酯（DLM）是一种常用于保护农作物免受害虫侵害的杀虫剂。QT间期延长伴恶性室性心律失常是最常见的心血管并发症之一。DLM中毒会导致抗氧化酶水平降低。谷氨酰胺是谷胱甘肽的前体，谷胱甘肽是一种抗氧化剂，已被证明可改善多种危重病后的预后。
我们假设谷氨酰胺通过其抗氧化特性，可能拮抗DLM的心脏毒性作用。
所有实验均在8周龄雄性Wistar白化大鼠身上进行。大鼠被分为以下几组（n = 10）；第一组：对照组，第二组：L-谷氨酰胺组，第三组：DLM组，第四组：DLM组且在4小时后给予L-谷氨酰胺。对心脏组织进行总抗氧化状态（TAS）、总氧化状态（TOS）及参数分析。
我们发现DLM组的TAS较高而TOS较低。我们还发现DLM组间质水肿和炎性细胞浸润明显更频繁，且DLM组的QT和QTc高于其他组。
最近的研究表明，几种特殊氨基酸，如谷氨酰胺、甘氨酸、精氨酸和牛磺酸，对心肌细胞具有细胞保护作用，并已证实谷氨酰胺具有心脏保护特性。
在本研究中，我们展示了谷氨酰胺对大鼠DLM心脏毒性作用的保护作用。通过显示氧化应激标志物的组织水平改善以及QT间期延长的改善，证实了这种保护作用。
