Schulz T F, Scharfenberger H, Lambris J D, Rieber P, Riethmüller G, Dierich M P
Immunology. 1985 Apr;54(4):791-800.
A monoclonal antibody, M522, reacting with human monocytes, neutrophils and a proportion of non-adherent PBL in a pattern similar to OKM-1 and anti-Mo-1, and precipitating a dimer of MW 165,000 and 100,000 from neutrophils, was shown to react with C3 at an epitope localized on the alpha-chain of C3. F(ab)2-fragments of M522 stimulate the respiratory burst of neutrophils and monocytes. M522 differs from two monoclonal antibodies against the Mo-1 molecule with respect to the capacity to inhibit the binding of sheep erythrocytes coated with different C3-fragments to C-receptor carrying cells. It inhibited the binding of H-coated particles to B-lymphoid cells and precipitated a 50,000 MW molecule from RAJI cells and tonsil lymphocytes. The results obtained suggest an antigenic relationship between the alpha-chain of C3, the heavy chain of a membrane molecule involved in neutrophil/monocyte activation, and a B-cell molecule of MW 50,000.
一种单克隆抗体M522，它与人类单核细胞、中性粒细胞以及一部分非贴壁外周血淋巴细胞发生反应，其反应模式类似于OKM-1和抗Mo-1，并能从嗜中性粒细胞中沉淀出分子量为165,000和100,000的二聚体，已证明它在C3α链上的一个表位与C3发生反应。M522的F(ab)2片段可刺激中性粒细胞和单核细胞的呼吸爆发。在抑制包被不同C3片段的绵羊红细胞与携带C受体的细胞结合的能力方面，M522与两种抗Mo-1分子的单克隆抗体不同。它抑制H包被颗粒与B淋巴细胞的结合，并从RAJI细胞和扁桃体淋巴细胞中沉淀出一个分子量为50,000的分子。所得结果表明C3α链、参与中性粒细胞/单核细胞激活的膜分子重链以及一个分子量为50,000的B细胞分子之间存在抗原关系。
