Dron M, Tovey M G, Eid P
J Gen Virol. 1985 Apr;66 ( Pt 4):787-95. doi: 10.1099/0022-1317-66-4-787.
The pattern of both constitutive and interferon-induced proteins was determined by two-dimensional gel electrophoresis in parental and interferon-resistant clones of Daudi cells in relation to the phenotype of interferon resistance. The complement of constitutive proteins present in clones DIF3, DIF8, DIF9 and DIF10 appeared to be identical to that of parental Daudi cells even though these cells were resistant to both the antiviral and anti-proliferative actions of interferon. Treatment of Daudi cells for 20 h with 10(3) reference units/ml of electrophoretically pure human interferon-alpha resulted in the induction of 15 proteins of molecular weights ranging from 15000 to 62000 detected after a 4 h labelling period with L-[35S]methionine. A number of these proteins were also induced in interferon-resistant clones of Daudi cells although some of these proteins appeared later and in smaller amounts than in the interferon-treated parental cells. However, seven proteins with molecular weights ranging from 18000 to 58000 which were induced by interferon in parental Daudi cells were not induced in any of the four interferon-resistant clones, suggesting that the phenotype of interferon resistance of these cells may be related to a reduction or absence of certain interferon-induced protein(s).
通过二维凝胶电泳法，在与干扰素抗性表型相关的Daudi细胞亲本克隆和干扰素抗性克隆中，确定组成型蛋白和干扰素诱导蛋白的模式。克隆DIF3、DIF8、DIF9和DIF10中存在的组成型蛋白补充物似乎与亲本Daudi细胞的相同，尽管这些细胞对干扰素的抗病毒和抗增殖作用具有抗性。用10³参考单位/毫升的电泳纯人干扰素-α处理Daudi细胞20小时，在用L-[³⁵S]甲硫氨酸标记4小时后，诱导出15种分子量范围从15000到62000的蛋白质。Daudi细胞的干扰素抗性克隆中也诱导出了许多这些蛋白质，尽管其中一些蛋白质出现的时间比经干扰素处理的亲本细胞晚，且数量较少。然而，在亲本Daudi细胞中由干扰素诱导的7种分子量范围从18000到58000的蛋白质，在四个干扰素抗性克隆中均未被诱导，这表明这些细胞的干扰素抗性表型可能与某些干扰素诱导蛋白的减少或缺失有关。
