Murphy K A, Unruh T R, Zhou L M, Zalom F G, Shearer P W, Beers E H, Walton V M, Miller B, Chiu J C
Department of Entomology and Nematology,College of Agricultural and Environmental Sciences,University of California,Davis,CA 95616,USA.
USDA-ARS,Wapato,WA 98951,USA.
Bull Entomol Res. 2015 Jun;105(3):364-72. doi: 10.1017/S0007485315000218. Epub 2015 Mar 25.
Drosophila suzukii (Spotted Wing Drosophila) has recently become a serious invasive pest of fruit crops in the USA, Canada, and Europe, leading to substantial economic losses. D. suzukii is a direct pest, ovipositing directly into ripe or ripening fruits; in contrast, other Drosophilids utilize decaying or blemished fruits and are nuisance pests at worst. Immature stages of D. suzukii are difficult to differentiate from other Drosophilids, posing problems for research and for meeting quarantine restrictions designed to prevent the spread of this pest in fruit exports. Here we used a combined phylogenetic and bioinformatic approach to discover genetic markers suitable for a species diagnostic protocol of this agricultural pest. We describe a molecular diagnostic for rapid identification of single D. suzukii larva using multiplex polymerase chain reaction. Our molecular diagnostic was validated using nine different species of Drosophila for specificity and 19 populations of D. suzukii from different geographical regions to ensure utility within species.
铃木果蝇（斑翅果蝇）最近已成为美国、加拿大和欧洲水果作物的一种严重入侵害虫，造成了巨大的经济损失。铃木果蝇是一种直接害虫，直接在成熟或正在成熟的果实中产卵；相比之下，其他果蝇类利用腐烂或有瑕疵的果实，充其量只是令人讨厌的害虫。铃木果蝇的未成熟阶段很难与其他果蝇类区分开来，这给研究以及满足旨在防止这种害虫在水果出口中传播的检疫限制带来了问题。在这里，我们采用系统发育和生物信息学相结合的方法来发现适合这种农业害虫物种诊断方案的遗传标记。我们描述了一种使用多重聚合酶链反应快速鉴定单个铃木果蝇幼虫的分子诊断方法。我们的分子诊断方法针对9种不同的果蝇进行了特异性验证，并对来自不同地理区域的19个铃木果蝇种群进行了验证，以确保在物种内的实用性。
