Song Guodong, Wu Yan, Wang Fang, Shao Yang, Jiang Jinzhu, Fan Chunjie, Li Peilong, Zhang Yonghu, Zuo Haibin
Department of Burns, Jinan Central Hospital Affiliated to Shandong University, 105 Jiefang Road, Jinan, 250013, Shandong, People's Republic of China,
J Mater Sci Mater Med. 2015 Apr;26(4):170. doi: 10.1007/s10856-015-5503-6. Epub 2015 Mar 25.
Acellular dermal matrix (ADM) has been widely used in repair and reconstruction of tissue defect. Therapeutic effect of porcine ADM (PADM) is inferior to that of human ADM (HADM). Relatively high immunogenicity and the resulting strong inflammatory response are major issue in application of PADM. We therefore treated reticular layer PADM (Rl-PADM) with matrix metalloproteinase-7 (MMP-7) and obtained a low-immunogenicity porcine dermal scaffold (LIPDS). Highly immunogenic components, tissue structure, cytocompatibility, and postgrafting histological changes of LIPDS were further investigated. Compared with Rl-PADM, LIPDS showed that the epithelial root sheath, cell debris, laminin, and type IV collagen were almost entirely removed, the structure remained normal, and the interfibrous space was relatively enlarged. Cytocompatibility of LIPDS was similar to that of HADM but superior to Rl-PADM. With regard to the extent of tissue ingrowth in terms of host fibroblasts infiltration and vascularization, LIPDS exhibited clear advantages over Rl-PADM after they had been subcutaneously transplanted in a rat model. In addition, no excessive inflammatory response was observed in LIPDS group up to 28 days postgraft, and the morphosis of collagenous fibers kept essentially normal. However, there were stronger inflammatory response and obvious collagen spallation in Rl-PADM group. The processes of integration and remodeling after the LIPDS grafting were similar to those of a normal wound healing response. The LIPDS graft was vascularized at a relatively high speed. Thus, as an implantable scaffold material, LIPDS is a superior template for guiding tissue regeneration and remodeling.
脱细胞真皮基质（ADM）已广泛应用于组织缺损的修复与重建。猪脱细胞真皮基质（PADM）的治疗效果逊于人类脱细胞真皮基质（HADM）。相对较高的免疫原性以及由此引发的强烈炎症反应是PADM应用中的主要问题。因此，我们用基质金属蛋白酶-7（MMP-7）处理网状层PADM（Rl-PADM），获得了一种低免疫原性的猪真皮支架（LIPDS）。进一步研究了LIPDS的高免疫原性成分、组织结构、细胞相容性以及移植后的组织学变化。与Rl-PADM相比，LIPDS显示上皮根鞘、细胞碎片、层粘连蛋白和IV型胶原几乎完全去除，结构保持正常，纤维间空间相对增大。LIPDS的细胞相容性与HADM相似，但优于Rl-PADM。在大鼠模型中皮下移植后，就宿主成纤维细胞浸润和血管化方面的组织长入程度而言，LIPDS比Rl-PADM表现出明显优势。此外，LIPDS组在移植后28天内未观察到过度炎症反应，胶原纤维形态基本保持正常。然而，Rl-PADM组有更强的炎症反应和明显的胶原剥脱。LIPDS移植后的整合和重塑过程与正常伤口愈合反应相似。LIPDS移植物血管化速度相对较快。因此，作为一种可植入的支架材料，LIPDS是引导组织再生和重塑的优质模板。
