Cao Ruihua, Huang Fei, Wang Peihuan, Chen Chen, Zhu Guoxiong, Chen Lei, Wu Gaoyi
Department of Dermatology, The General Hospital of Jinan Military Commend, No. 25, Shi Fan Road, Jinan 250031, China.
Department of Stomatology, PLA Navy General Hospital, Beijing 100048, China.
Br J Oral Maxillofac Surg. 2015 May;53(5):430-5. doi: 10.1016/j.bjoms.2015.02.011. Epub 2015 Mar 22.
To investigate the changes in myosin heavy chain (MyHC) isoforms of rat masseter muscle fibres caused by chronic sleep deprivation and a possible link with the pathogenesis of disorders of the temporomandibular joint (TMJ). A total of 180 male rats were randomly divided into three groups (n=60 in each): cage controls, large platform controls, and chronic sleep deprivation group. Each group was further divided into three subgroups with different observation periods (7, 14, and 21 days). We investigated he expression of MyHC isoforms in masseter muscle fibres by real-time quantitative polymerase chain reaction (PCR), Western blotting, and immunohistochemical staining. In rats with chronic sleep deprivation there was increased MyHC-I expression in layers of both shallow and deep muscles at 7 and 21 days compared with the control groups, whereas sleep deprivation was associated with significantly decreased MyHC-II expression. At 21 days, there were no differences in MyHC-I or MyHC-II expression between the groups and there were no differences between the two control groups at any time point. These findings suggest that chronic sleep deprivation alters the expression of MyHC isoforms, which may contribute to the pathogenesis of disorders of the TMJ.
研究慢性睡眠剥夺引起的大鼠咬肌纤维肌球蛋白重链（MyHC）亚型变化以及与颞下颌关节（TMJ）紊乱发病机制的可能联系。将180只雄性大鼠随机分为三组（每组n = 60）：笼养对照组、大平台对照组和慢性睡眠剥夺组。每组再根据不同观察期（7、14和21天）分为三个亚组。通过实时定量聚合酶链反应（PCR）、蛋白质免疫印迹法和免疫组织化学染色，研究咬肌纤维中MyHC亚型的表达。与对照组相比，慢性睡眠剥夺大鼠在第7天和第21天浅层和深层肌肉层中MyHC-I表达增加，而睡眠剥夺与MyHC-II表达显著降低有关。在第21天，各组之间MyHC-I或MyHC-II表达无差异，两个对照组在任何时间点也无差异。这些结果表明，慢性睡眠剥夺会改变MyHC亚型的表达，这可能有助于TMJ紊乱的发病机制。
