clinorotation对骨髓间充质干细胞增殖和分化的影响及机制。

The effects and mechanisms of clinorotation on proliferation and differentiation in bone marrow mesenchymal stem cells.

作者信息

Yan Ming, Wang Yongchun, Yang Min, Liu Yanwu, Qu Bo, Ye Zhengxu, Liang Wei, Sun Xiqing, Luo Zhuojing

机构信息

Department of Orthopaedic Surgery, XiJing Hospital, The Fourth Military Medical University, Xi'an 710032, China.

Department of Aerospace Biodynamics, School of Aerospace Medicine, Fourth Military Medical University, Xi'an 710032, China.

出版信息

Biochem Biophys Res Commun. 2015 May 1;460(2):327-32. doi: 10.1016/j.bbrc.2015.03.034. Epub 2015 Mar 21.

DOI:10.1016/j.bbrc.2015.03.034

PMID:25804637

Abstract

Data from human and rodent studies have demonstrated that microgravity induces observed bone loss in real spaceflight or simulated experiments. The decrease of bone formation and block of maturation may play important roles in bone loss induced by microgravity. The aim of this study was to investigate the changes of proliferation and differentiation in bone marrow mesenchymal stem cells (BMSCs) induced by simulated microgravity and the mechanisms underlying it. We report here that clinorotation, a simulated model of microgravity, decreased proliferation and differentiation in BMSCs after exposure to 48 h simulated microgravity. The inhibited proliferation are related with blocking the cell cycle in G2/M and enhancing the apoptosis. While alterations of the osteoblast differentiation due to the decreased SATB2 expression induced by simulated microgravity in BMSCs.

摘要

来自人类和啮齿动物研究的数据表明，在实际太空飞行或模拟实验中，微重力会导致明显的骨质流失。骨形成的减少和成熟的阻滞可能在微重力诱导的骨质流失中起重要作用。本研究的目的是探讨模拟微重力诱导的骨髓间充质干细胞（BMSC）增殖和分化的变化及其潜在机制。我们在此报告，clinorotation（一种微重力模拟模型）在暴露于48小时模拟微重力后，会降低BMSC的增殖和分化。增殖受抑制与细胞周期在G2/M期受阻以及细胞凋亡增加有关。而BMSC中模拟微重力诱导的SATB2表达降低导致成骨细胞分化发生改变。