Takabayashi Shuji, Katoh Hideki
Experimental Animals Institute, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan,
Primates. 2015 Jul;56(3):235-40. doi: 10.1007/s10329-015-0466-2. Epub 2015 Mar 25.
The common marmoset (Callithrix jacchus) is a New World primate that is a useful model for medical studies. In this study, we report a convenient, reliable, and noninvasive procedure to genotype a living common marmoset by using fingernails. This method was used to successfully genotype DNA by restriction fragment length polymorphism (RFLP) PCR without prior purification, by using the KOD FX PCR enzyme kit. Additionally, there is no sample contamination from hematopoietic chimera derived from fused placenta in utero. We compared chimeric levels between various tissues in females with male littermates using quantitative fluorescent (QF)-PCR to prepare a reliable DNA source for genetic analyses, such as genotyping, gene mapping, or genomic sequencing. The chimerism detected appeared to be restricted to lymphatic tissues, such as bone marrow, thymus, spleen, lymph nodes and blood cells. As a result, DNA from fingernails with the quick is the best DNA source for genetic research in living marmosets.
普通狨猴（Callithrix jacchus）是一种新大陆灵长类动物，是医学研究的有用模型。在本研究中，我们报告了一种通过使用指甲对活体普通狨猴进行基因分型的便捷、可靠且非侵入性的方法。该方法通过使用KOD FX PCR酶试剂盒，无需事先纯化，即可通过限制性片段长度多态性（RFLP）PCR成功对DNA进行基因分型。此外，不存在来自子宫内融合胎盘产生的造血嵌合体的样本污染。我们使用定量荧光（QF）-PCR比较了雌性与雄性同窝幼仔不同组织之间的嵌合水平，以制备用于基因分型、基因图谱绘制或基因组测序等遗传分析的可靠DNA来源。检测到的嵌合现象似乎仅限于骨髓、胸腺、脾脏、淋巴结和血细胞等淋巴组织。因此，快速获取的指甲DNA是活体狨猴遗传研究的最佳DNA来源。
