School of Forensic Medicine, China Medical University, Shenyang 110001, Liaoning Province, China ; School of Forensic Medicine, China Criminal Police University, Shenyang 110035, Liaoning Province, China.
Department of Chemistry, China Medical University School of Basic Medicine, Shenyang 110001, Liaoning Province, China.
Neural Regen Res. 2012 Feb 5;7(4):290-4. doi: 10.3969/j.issn.1673-5374.2012.04.009.
In this study, we sought to elucidate the process of DNA degradation in brain and dental pulp cells of mice, within postmortem 0-72 hours, by using the single cell gel electrophoresis assay and professional comet image analysis and processing techniques. The frequency of comet-like cells, the percentage of tail DNA, tail length, tail moment, Olive moment and tail area increased in tandem with increasing postmortem interval. In contrast, the head radius, the percentage of head DNA and head area showed a decreasing trend. Linear regression analysis revealed a high correlation between these parameters and the postmortem interval. The findings suggest that the single cell gel electrophoresis assay is a quick and sensitive method to detect DNA degradation in brain and dental pulp cells, providing an objective and accurate new way to estimate postmortem interval.
在这项研究中，我们通过单细胞凝胶电泳检测和专业彗星图像分析处理技术，旨在阐明死后 0-72 小时内小鼠大脑和牙髓细胞中 DNA 降解的过程。彗星样细胞的频率、尾部 DNA 的百分比、尾部长度、尾部矩、Olive 矩和尾部面积与死后间隔时间呈正相关。相比之下，头部半径、头部 DNA 的百分比和头部面积呈下降趋势。线性回归分析显示这些参数与死后间隔时间之间存在高度相关性。研究结果表明，单细胞凝胶电泳检测是一种快速、敏感的方法，可用于检测大脑和牙髓细胞中的 DNA 降解，为估计死后间隔时间提供了一种客观、准确的新方法。
