促红细胞生成素上调生长相关蛋白-43 的表达，并促进视神经挤压后体内视网膜神经节细胞轴突的再生。
Erythropoietin upregulates growth associated protein-43 expression and promotes retinal ganglion cell axonal regeneration in vivo after optic nerve crush.
机构信息
Department of Ophthalmology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Department of Clinical Pharmacology, Changhai Hospital Affiliated to Second Military Medical University, Shanghai 200433, China.
出版信息
Neural Regen Res. 2012 Feb 5;7(4):295-301. doi: 10.3969/j.issn.1673-5374.2012.04.010.
In this study, we established a rat model of optic nerve crush to explore the effects of erythropoietin on retinal ganglion cell axonal regeneration. At 15 days after injury in erythropoietin treated rats, retinal ganglion cell densities in regions corresponding to the 1/6, 3/6 and 5/6 ratios of the retinal radius were significantly increased. In addition, the number of growth associated protein-43 positive axons was significantly increased at different distances (50, 250 and 500 μm) from the crush site after erythropoietin treatment. Erythropoietin significantly increased growth associated protein-43 protein levels in the retina after crush injury, as determined by western blot and immunofluorescence analysis. These results demonstrate that erythropoietin protects injured retinal ganglion cells and promotes axonal regeneration.
在这项研究中，我们建立了视神经挤压大鼠模型，以探讨促红细胞生成素对视网膜神经节细胞轴突再生的影响。在损伤后 15 天，促红细胞生成素治疗组大鼠对应视网膜半径 1/6、3/6 和 5/6 比例处的视网膜神经节细胞密度显著增加。此外，在促红细胞生成素治疗后，不同距离（挤压部位 50、250 和 500μm）处生长相关蛋白-43 阳性轴突的数量显著增加。Western blot 和免疫荧光分析表明，促红细胞生成素可显著增加挤压伤后视网膜中生长相关蛋白-43 蛋白水平。这些结果表明，促红细胞生成素可保护损伤的视网膜神经节细胞并促进轴突再生。
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