Pukkila P J, Lu B C
Chromosoma. 1985;91(2):108-12. doi: 10.1007/BF00294053.
We have taken advantage of the synchronous meiotic process in the basidiomycete Coprinus cinereus to develop a simple and rapid method to selectively stain meiotic chromosomes and nucleoli in this fungus without prior removal of the cell wall. Electron microscopic examination of these silver-stained chromosomes indicated that the lateral elements of the synaptonemal complexes were prominently stained, and terminal attachment plaques were apparent. We found that a translocation quadrivalent could be recognized easily in the light microscope using these methods. The procedures appear suitable for the characterization of chromosome rearrangements in this small genome, and should facilitate cytogenetic analysis in this fungus.
我们利用担子菌灰盖鬼伞的同步减数分裂过程，开发了一种简单快速的方法，可在不预先去除细胞壁的情况下，选择性地对这种真菌的减数分裂染色体和核仁进行染色。对这些银染染色体的电子显微镜检查表明，联会复合体的侧生元件被显著染色，并且末端附着斑明显可见。我们发现，使用这些方法可以在光学显微镜下轻松识别易位四价体。这些程序似乎适用于对这个小基因组中的染色体重排进行表征，并且应该有助于对这种真菌进行细胞遗传学分析。
