Kent J E, Blackmore D J
Equine Vet J. 1985 Mar;17(2):125-9. doi: 10.1111/j.2042-3306.1985.tb02065.x.
This paper describes the quantitative measurement of IgG concentrations in equine blood/serum by turbidimetric analysis and the qualitative assessment using latex agglutination. The concentration of IgG in foal serum measured by turbidimetry correlated well with the results obtained by radial immunodiffusion (r = 0.91) and the gamma-globulins estimated from the electrophoresis of serum on cellulose acetate (r = 0.92). The method, using antibody-coated latex beads, to detect foals with serum IgG concentrations of less than 4 g/litre (whole blood less than 2 g/litre) proved to be accurate in 96 per cent of cases and provided a quick and simple qualitative test, suitable for use outside the laboratory.
本文描述了通过比浊法对马血/血清中IgG浓度进行定量测定以及使用乳胶凝集法进行定性评估。通过比浊法测定的马驹血清中IgG浓度与通过放射免疫扩散法获得的结果相关性良好(r = 0.91),并且与从醋酸纤维素上的血清电泳估计的γ-球蛋白相关性良好(r = 0.92)。使用包被抗体的乳胶珠检测血清IgG浓度低于4克/升(全血低于2克/升)的马驹的方法在96%的病例中被证明是准确的,并且提供了一种快速简单的定性检测方法,适用于实验室外使用。