Eckersall P D, Conner J G, Harvie J
Department of Veterinary Clinical Biochemistry, University of Glasgow Veterinary School, UK.
Vet Res Commun. 1991;15(1):17-24. doi: 10.1007/BF00497786.
Antiserum was raised in sheep against canine C-reactive protein (CRP) and antibody, which was not specific for CRP, was removed by absorption with normal canine serum protein linked to agarose beads. The antiserum was used to develop an immunoturbidimetric assay for canine CRP on a MIRA (Roche Diagnostics) automated clinical biochemical analyser and assessed for routine analysis of CRP in canine serum samples. The assay gave standard curves with each standard having a coefficient of variance (CV) between 4.8 and 11%, interassay CVs below 11% and intra-assay CVs of less than 5%. Parallel dilution curves were obtained with purified CRP diluted in buffer and with endogenous CRP in serum diluted with buffer or with a serum with a negligible CRP content. The immunoturbidimetric assay results correlated with the results obtained using an ELISA method, r = 0.88. The immunoturbidimetric assay of canine CRP proved to be suitable for the routine analysis of canine CRP.
用犬C反应蛋白（CRP）对绵羊进行免疫，制备抗血清，并通过与琼脂糖珠偶联的正常犬血清蛋白吸收去除非特异性针对CRP的抗体。该抗血清用于在MIRA（罗氏诊断）自动临床生化分析仪上开发犬CRP免疫比浊法，并用于犬血清样本中CRP的常规分析评估。该检测方法给出了标准曲线，每个标准品的变异系数（CV）在4.8%至11%之间，批间CV低于11%，批内CV小于5%。用缓冲液稀释的纯化CRP以及用缓冲液或CRP含量可忽略不计的血清稀释的血清中的内源性CRP获得了平行稀释曲线。免疫比浊法检测结果与ELISA法结果相关，r = 0.88。犬CRP免疫比浊法被证明适用于犬CRP的常规分析。
