Redshaw J D, Bisby M A
Exp Neurol. 1985 May;88(2):437-46. doi: 10.1016/0014-4886(85)90205-5.
Proteins of fast axonal transport in rat sciatic nerve axons were separated and characterized by SDS-polyacrylamide gel electrophoresis and fluorography, after injection of L-[35S]methionine into the dorsal root ganglion. The effects of crushing or resecting the sciatic nerve on the relative labeling of specific polypeptide bands were compared. Initially, both types of axon injury produced the same response, but after 3 weeks there was a partial return to normal composition in crushed nerves. In resected nerves, the changes characteristic of axon injury persisted beyond 7 weeks. Behavioral testing showed that crushed nerves reinnervated foot skin, whereas no reinnervation was detected after resection. We conclude that in sensory neurons, as in several other neuronal types, the restoration in normal composition of fast-transported protein after axon injury depends on reinnervation of target tissues. This aspect of the cell body reaction to injury seems to be regulated by a retrograde trophic interaction with the target.
将L-[35S]甲硫氨酸注入背根神经节之后，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和荧光自显影对大鼠坐骨神经轴突中快速轴突运输的蛋白质进行分离和鉴定。比较了挤压或切断坐骨神经对特定多肽带相对标记的影响。最初，两种类型的轴突损伤产生相同的反应，但3周后，挤压神经中的成分部分恢复正常。在切断的神经中，轴突损伤的特征性变化持续超过7周。行为测试表明，挤压神经能重新支配足部皮肤，而切断神经后未检测到重新支配。我们得出结论，在感觉神经元中，与其他几种神经元类型一样，轴突损伤后快速运输蛋白正常成分的恢复取决于靶组织的重新支配。细胞体对损伤反应的这一方面似乎受与靶标的逆行营养相互作用调节。
