体外暴露于巨细胞病毒或爱泼斯坦-巴尔病毒后人白细胞中的干扰素诱导作用。

Interferon induction in human leukocytes after in vitro exposure to cytomegalovirus or Epstein-Barr virus.

作者信息

Einhorn L, Einhorn S, Wahren B

出版信息

Intervirology. 1985;23(3):140-9. doi: 10.1159/000149597.

Abstract

Interferon (IFN) was produced after exposure of human mononuclear leukocytes and bone marrow cells to infectious or noninfectious cytomegalovirus (CMV) in vitro. The IFN was generated mainly by non-B lymphocytes. Both alpha- and gamma-type IFN could be demonstrated. CMV antigens were usually not demonstrable in CMV-exposed leukocytes. Addition of anti-IFN antibodies did not induce CMV antigens. Thus, it seems that the endogenous production of IFN is not responsible for the difficulties in demonstrating CMV antigens after in vitro exposure of normal human leukocytes to CMV. Addition of Epstein-Barr virus (EBV) B95-8 to leukocytes induced the production of alpha-type IFN. Exogenously added IFN reduced the induction of EBV-determined nuclear antigen (EBNA). However, the presence of anti-IFN antibodies in EBV-infected cultures did not increase the number of EBNA-positive cells.

摘要

在体外将人单核白细胞和骨髓细胞暴露于感染性或非感染性巨细胞病毒（CMV）后可产生干扰素（IFN）。IFN主要由非B淋巴细胞产生。可检测到α型和γ型IFN。在暴露于CMV的白细胞中通常检测不到CMV抗原。添加抗IFN抗体不会诱导CMV抗原产生。因此，似乎内源性IFN的产生并非导致正常人白细胞在体外暴露于CMV后难以检测到CMV抗原的原因。将爱泼斯坦-巴尔病毒（EBV）B95-8添加到白细胞中可诱导α型IFN的产生。外源性添加的IFN可减少EBV决定的核抗原（EBNA）的诱导。然而，在EBV感染的培养物中存在抗IFN抗体并不会增加EBNA阳性细胞的数量。