Oh Kwang-Im, Smith-Dupont Kathryn B, Markiewicz Beatrice N, Gai Feng
Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104.
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139.
Biopolymers. 2015 Jul;104(4):281-90. doi: 10.1002/bip.22640.
Despite our extensive understanding of water-soluble protein folding kinetics, much less is known about the folding dynamics and mechanisms of membrane proteins. However, recent studies have shown that for relatively simple systems, such as peptides that form a transmembrane α-helix, helical dimer, or helix-turn-helix, it is possible to assess the kinetics of several important steps, including peptide binding to the membrane from aqueous solution, peptide folding on the membrane surface, helix insertion into the membrane, and helix-helix association inside the membrane. Herein, we provide a brief review of these studies and also suggest new initiation and probing methods that could lead to improved temporal and structural resolution in future experiments.
尽管我们对水溶性蛋白质折叠动力学有广泛的了解，但对膜蛋白的折叠动力学和机制了解得要少得多。然而，最近的研究表明，对于相对简单的系统，如形成跨膜α螺旋、螺旋二聚体或螺旋-转角-螺旋的肽，有可能评估几个重要步骤的动力学，包括肽从水溶液与膜结合、肽在膜表面折叠、螺旋插入膜以及膜内螺旋-螺旋缔合。在此，我们简要回顾这些研究，并提出新的起始和探测方法，这些方法可能会在未来的实验中提高时间和结构分辨率。
