Silva Renan O, Damasceno Samara R B, Brito Tarcísio V, Dias Jordana M, Fontenele Amanda M, Braúna Isabela S, Júnior José S C, Maciel Jeanny S, de Paula Regina C M, Ribeiro Ronaldo A, Souza Marcellus H L P, Freitas Ana L P, Medeiros Jand-Venes R, Silva Draulio C, Barbosa André L R
Laboratory of Pharmacology of Inflammation and Cancer, Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, Ceará, Brazil.
Laboratory of Experimental Physiopharmacology, Biotechnology and Biodiversity Center Research (BIOTEC), Federal University of Piauí, Parnaíba, Piauí, Brazil.
J Pharm Pharmacol. 2015 Jul;67(7):1017-27. doi: 10.1111/jphp.12399. Epub 2015 Mar 24.
The aim of the study was to investigate the anti-inflammatory, antioxidant and antinociceptive actions of PFPe, a polysaccharide fraction isolated from the dried fruit of the Passiflora edulis.
Animals were pretreated with PFPe (0.3, 1 or 3 mg/kg, i.p.) 1 h before induction of paw oedema by carrageenan, histamine, serotonin, compound 48/80 or prostaglandin E2 (PGE2). Neutrophil migration and vascular permeability were measured after carrageenan injection into the peritoneum, and the action of the PFPe on the tumour necrosis factor-alpha, interleukin-1 beta (IL-1β), myeloperoxidase (MPO), glutathione (GSH) and malondialdehyde (MDA) levels was also evaluated. To assay nociception, we examined acetic acid-induced writhing, formalin-induced paw licking and response latency in the hot plate test.
Pretreatment with PFPe significantly inhibited carrageenan-induced paw oedema. PFPe also reduced paw oedema induced by compound 48/80, histamine, serotonin, and PGE2 and compound 48/80-induced vascular permeability. In addition, PFPe significantly reduced the MPO activity, MDA and GSH concentrations, and IL-1β level. In the nociception tests, PFPe reduced acetic acid-induced writhing and formalin-induced paw licking and did not increase the response latency time.
Our results suggest that PFPe administration reduces the inflammatory response by modulation of the liberation or synthesis of histamine and serotonin, by reduction of neutrophil migration, IL-1β levels, and oxidative stress and nociception.
本研究旨在探讨从西番莲干燥果实中分离得到的多糖组分PFPe的抗炎、抗氧化和抗伤害感受作用。
在通过角叉菜胶、组胺、5-羟色胺、化合物48/80或前列腺素E2（PGE2）诱导爪部水肿前1小时，用PFPe（0.3、1或3mg/kg，腹腔注射）对动物进行预处理。向腹膜内注射角叉菜胶后，测量中性粒细胞迁移和血管通透性，并评估PFPe对肿瘤坏死因子-α、白细胞介素-1β（IL-1β）、髓过氧化物酶（MPO）、谷胱甘肽（GSH）和丙二醛（MDA）水平的作用。为了测定痛觉感受，我们检测了乙酸诱导的扭体反应、福尔马林诱导的舔爪反应以及热板试验中的反应潜伏期。
PFPe预处理可显著抑制角叉菜胶诱导的爪部水肿。PFPe还可减轻化合物48/80、组胺、5-羟色胺和PGE2诱导的爪部水肿以及化合物48/80诱导的血管通透性。此外，PFPe可显著降低MPO活性、MDA和GSH浓度以及IL-1β水平。在痛觉感受试验中，PFPe可减少乙酸诱导的扭体反应和福尔马林诱导的舔爪反应，且不增加反应潜伏期时间。
我们的结果表明，给予PFPe可通过调节组胺和5-羟色胺的释放或合成、减少中性粒细胞迁移、IL-1β水平、氧化应激和痛觉感受来减轻炎症反应。
