Golding H, Bluestone J, Satz M L, Singer D, Singer A
J Immunol. 1985 Jun;134(6):3557-9.
This communication describes in vitro culture conditions that permit the use of MHC gene-transfected L cells as stimulators of primary CTL responses. The use of L cells as stimulators usually results in the generation of "nonspecific" anti-L cell cytotoxicity too great to permit visualization of antigen-specific CTL responses. This study demonstrates that the generation of such anti-L cell cytotoxicity can be abrogated by strategies that prevent the in vitro activation of self-Ia-restricted TH cells. These maneuvers allowed the use of L cell transfectants as stimulators of allospecific and xenospecific CTL precursors in primary in vitro mixed lymphocyte reaction cultures.
本通讯描述了体外培养条件，该条件允许使用MHC基因转染的L细胞作为初次CTL反应的刺激物。使用L细胞作为刺激物通常会导致产生“非特异性”抗L细胞细胞毒性，其程度过大以至于无法观察到抗原特异性CTL反应。本研究表明，通过防止自身Ia限制的TH细胞在体外活化的策略，可以消除这种抗L细胞细胞毒性的产生。这些操作使得L细胞转染体能够在初次体外混合淋巴细胞反应培养物中作为同种特异性和异种特异性CTL前体的刺激物。
