Eccles S J, Teh H S, Diamond A G, McMaster W R
Immunology. 1986 Sep;59(1):29-35.
A cosmid (cos a 13.1) containing RT1 class II B alpha and B beta genes was introduced into mouse and rat fibroblast cell lines by transformation. Mouse L cells transformed with cos a 13.1-synthesized cell surface class II molecules that were similar, with respect to apparent molecular weight and binding of xenogeneic- and allogeneic-specific antibodies, to class II molecules on rat B cells. RT1 class II molecules on the surface of mouse L cells were recognized by both allogeneic and xenogeneic T cells. In contrast, rat-2 cells transformed with cos a 13.1 did not synthesize any detectable RT1 class II molecules at the cell surface, and the levels of B alpha and B beta mRNA were generally very low or undetectable. This differential expression of exogenous class II genes was not exclusively due to a trans-acting positive regulatory factor in L cells. Other possible explanations for this difference are discussed.
通过转化将含有RT1 II类Bα和Bβ基因的黏粒（黏粒a 13.1）导入小鼠和大鼠成纤维细胞系。用黏粒a 13.1转化的小鼠L细胞合成了细胞表面II类分子，就表观分子量以及异种和同种异体特异性抗体的结合而言，这些分子与大鼠B细胞上的II类分子相似。小鼠L细胞表面的RT1 II类分子可被同种异体和异种T细胞识别。相比之下，用黏粒a 13.1转化的大鼠-2细胞在细胞表面未合成任何可检测到的RT1 II类分子，且Bα和Bβ mRNA水平通常非常低或无法检测到。外源II类基因的这种差异表达并非完全归因于L细胞中的反式作用阳性调节因子。文中讨论了造成这种差异的其他可能解释。
