Di Bartolo Ilaria, Angeloni Giorgia, Tofani Silvia, Monini Marina, Ruggeri Franco Maria
Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy,
Arch Virol. 2015 Jun;160(6):1533-6. doi: 10.1007/s00705-015-2397-z. Epub 2015 Mar 27.
Two-hundred eight swine fecal samples from six Italian farms were tested using a kobuvirus-specific RT-PCR with primers that amplify a region within the 3D gene. All farms were kobuvirus positive, with prevalence rates ranging between 24 % and 84 %. Overall, 57.5 % of asymptomatic pigs and 49.7 % of animals with diarrhea were positive for kobuvirus. Sequence analysis showed a different predominant strain circulating on each farm and indicated that the strains detected were related to both European and Asiatic strains. A possible pathogenic role of kobuvirus should be investigated further, since infections with this virus occur frequently in pigs of different ages.
使用针对杯状病毒的逆转录聚合酶链反应（RT-PCR）和能扩增3D基因内一个区域的引物，对来自六个意大利农场的208份猪粪便样本进行了检测。所有农场的猪杯状病毒检测均呈阳性，流行率在24%至84%之间。总体而言，57.5%的无症状猪和49.7%腹泻猪的杯状病毒检测呈阳性。序列分析表明，每个农场都有不同的优势毒株在传播，并且检测到的毒株与欧洲毒株和亚洲毒株均有关联。由于不同年龄的猪频繁感染这种病毒，因此应进一步研究杯状病毒可能的致病作用。
