Chuchaona Watchaporn, Khamrin Pattara, Yodmeeklin Arpaporn, Kongkaew Aphisek, Vachirachewin Ratchaya, Kumthip Kattareeya, Ushijima Hiroshi, Maneekarn Niwat
Department of Microbiology, Faculty of Medicine, Chiang Mai University, 110 Intawarorose, Sriphoom, Muang, Chiang Mai, 50200, Thailand.
Animal House Unit, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
Trop Anim Health Prod. 2017 Jun;49(5):1077-1080. doi: 10.1007/s11250-017-1298-4. Epub 2017 May 2.
A total of 636 fecal samples collected from piglets with and without diarrhea during 2009 to 2013 were tested for porcine kobuvirus by RT-PCR. From a total of 528 fecal samples collected from piglets with diarrhea and 108 from healthy controls, 505 (95.6%) and 104 (96.3%) were positive for porcine kobuvirus, respectively. The detection rates of porcine kobuvirus were remarkable equally high in both diarrheic and healthy piglets. Phylogenetic analysis revealed that porcine kobuvirus strains detected in both symptomatic and asymptomatic piglets were genetically closely related to each other and also to other porcine kobuviruses reported worldwide. It was interesting to point out that one of the porcine kobuvirus strains isolated from piglet in our study was similar to a porcine-like bovine kobuvirus reference strain isolated previously in South Korea. This finding provided the evidence to support the interspecies transmission of kobuviruses between cattle and swine.
2009年至2013年期间，共收集了636份腹泻仔猪和非腹泻仔猪的粪便样本，采用逆转录聚合酶链反应（RT-PCR）检测猪杯状病毒。从528份腹泻仔猪粪便样本和108份健康对照样本中，猪杯状病毒阳性样本分别为505份（95.6%）和104份（96.3%）。腹泻仔猪和健康仔猪中猪杯状病毒的检出率同样非常高。系统发育分析表明，有症状和无症状仔猪中检测到的猪杯状病毒株在基因上彼此密切相关，也与世界范围内报道的其他猪杯状病毒密切相关。值得指出的是，本研究中从仔猪分离出的一株猪杯状病毒与先前在韩国分离出的一株猪样牛杯状病毒参考株相似。这一发现为支持杯状病毒在牛和猪之间的种间传播提供了证据。
