纽约州高丛蓝莓中蓝莓焦枯病毒分离株的遗传变异性

Genetic variability of blueberry scorch virus isolates from highbush blueberry in New York State.

作者信息

Kalinowska Elżbieta, Marsella-Herrick Patricia, Fuchs Marc

机构信息

Department of Plath Pathology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, 159 Nowoursynowska Street, 02-776, Warsaw, Poland,

出版信息

Arch Virol. 2015 Jun;160(6):1537-42. doi: 10.1007/s00705-015-2402-6. Epub 2015 Mar 27.

DOI:10.1007/s00705-015-2402-6

PMID:25809019

Abstract

The genetic variability of blueberry scorch virus (BlScV) isolates from New York was determined within a portion of the RNA-dependent RNA polymerase gene and the triple gene block and coat protein (CP) genes. Phylogenetic analysis of 19 New York isolates and other isolates for which sequence information is available in GenBank revealed two distinct clades, regardless of the coding region analyzed, and limited variability within (0.029 ± 0.007) and between (0.183 ± 0.032) phylogroups. Recombination events were identified in the CP gene of three New York isolates, and codons of the five BlScV genes characterized were found to be under neutral or negative selective pressure.

摘要

对来自纽约的蓝莓焦枯病毒（BlScV）分离株的遗传变异性进行了测定，测定范围包括RNA依赖的RNA聚合酶基因的一部分、三基因块以及外壳蛋白（CP）基因。对19个纽约分离株以及GenBank中可获取序列信息的其他分离株进行系统发育分析，结果显示，无论分析的编码区域如何，均存在两个不同的进化枝，且进化枝内部（0.029±0.007）和进化枝之间（0.183±0.032）的变异性有限。在三个纽约分离株的CP基因中鉴定出重组事件，并且发现所表征的五个BlScV基因的密码子处于中性或负选择压力之下。