Emanuelsson H, Anehus S
J Embryol Exp Morphol. 1985 Feb;85:151-61.
In the polychaete Ophryotrocha labronica each oocyte is during its growth period associated with a single nurse cell. The fact that the oocyte-nurse cell pairs occur isolated in the female coelom makes them easily removable for analysis of their developmental ability in vitro. Using Dulbecco's modified Eagle medium supplemented with amino acids, nucleosides, foetal calf serum and sea water, we have managed to support development in vitro of germ cell pairs from early and mid-oogenesis until maturation of the oocyte, when the nurse cell degenerates and the oocyte enters meiotic metaphase. Radiolabelling of germ cells in mid-oogenesis with tritiated amino acids and uridine during the first day of incubation indicates normal development with synthesis of RNA and protein, and pulses two days later verify a continued normal protein synthesis and yolk formation. The investigation confirms autosynthesis of yolk proteins in the germ cells of this species and indicates a leading role of the nurse cell in the process.
在多毛纲动物拉氏奥费虫（Ophryotrocha labronica）中，每个卵母细胞在其生长期间都与单个滋养细胞相关联。卵母细胞 - 滋养细胞对孤立地存在于雌性体腔中，这使得它们易于移除，以便在体外分析其发育能力。使用添加了氨基酸、核苷、胎牛血清和海水的杜尔贝科改良伊格尔培养基，我们成功地支持了从早期和中期卵母细胞发生阶段的生殖细胞对在体外的发育，直至卵母细胞成熟，此时滋养细胞退化，卵母细胞进入减数分裂中期。在培养的第一天用氚标记的氨基酸和尿苷对中期卵母细胞发生阶段的生殖细胞进行放射性标记，表明其正常发育并合成RNA和蛋白质，两天后的脉冲验证了持续的正常蛋白质合成和卵黄形成。该研究证实了该物种生殖细胞中卵黄蛋白的自身合成，并表明滋养细胞在此过程中起主导作用。
