Heby O, Emanuelsson H
Med Biol. 1981 Dec;59(5-6):417-22.
The physiological function of the polyamines in germ cell differentiation and in early embryonic development was studied with the aid of the highly specific polyamine synthesis inhibitors DL-alpha-methylornithine (MO) and DL-alpha-difluoromethylornithine (DFMO). In the invertebrate Ophryotrocha labronica DFMO-induced polyamine deficiency severely affected nucleolar formation and activity in germ cell differentiation as revealed by RNA-labelling experiments and by ultrastructural analysis. Both in an invertebrate (Ophryotrocha labronica) and in a vertebrate (chick), inhibitor-induced polyamine deficiency caused a block of embryonic development at gastrulation. The major ultrastructural effect observed in the arrested embryos was an interference with nucleolar formation. Our data suggest that polyamine synthesis is essential for the ribosomal gene expression that is associated with oogenesis and embryogenesis.
借助高度特异性的多胺合成抑制剂DL-α-甲基鸟氨酸（MO）和DL-α-二氟甲基鸟氨酸（DFMO），研究了多胺在生殖细胞分化和早期胚胎发育中的生理功能。在无脊椎动物长吻沙蚕中，RNA标记实验和超微结构分析表明，DFMO诱导的多胺缺乏严重影响了生殖细胞分化中的核仁形成和活性。在无脊椎动物（长吻沙蚕）和脊椎动物（鸡）中，抑制剂诱导的多胺缺乏都会导致胚胎发育在原肠胚形成阶段受阻。在停滞胚胎中观察到的主要超微结构效应是对核仁形成的干扰。我们的数据表明，多胺合成对于与卵子发生和胚胎发生相关的核糖体基因表达至关重要。
