Health Secur. 2015 Jan-Feb;13(1):64-8. doi: 10.1089/hs.2014.0076. Epub 2015 Jan 28.
As an issue of biosecurity, it is important to identify the origin of a suspected sample to distinguish whether it originated from the release of a bioterrorism agent or from environmental contamination with a virulent agent. Here we have developed an analytical pipeline that can infer the phylogenetic position of Bacillus cereus group species, including B. anthracis, from next-generation sequencing reads without extensive genomics skills. GcoGSA-BA can also detect the existence of anthrax plasmid pXO1 carrying 3 anthrax toxins (lethal factor, edema factor, and protective antigen). This pipeline can also be used to correctly infer the phylogenetic position and to detect the suspected isolate carrying anthrax toxins among B. cereus group.
作为一个生物安全问题，确定疑似样本的来源很重要，以区分它是否源自生物恐怖主义制剂的释放，还是源自具有毒性制剂的环境污染。在这里，我们开发了一种分析管道，可以从下一代测序读取中推断出蜡样芽胞杆菌组物种的系统发育位置，包括炭疽芽孢杆菌，而无需广泛的基因组学技能。GcoGSA-BA 还可以检测携带 3 种炭疽毒素（致死因子、水肿因子和保护性抗原）的炭疽质粒 pXO1 的存在。该管道还可用于正确推断蜡样芽胞杆菌组中携带炭疽毒素的疑似分离株的系统发育位置。
