Pavone Chaine, Nogueira Andressa Vilas Boas, de Oliveira Guilherme José Pimentel Lopes, Scardueli Cássio Rocha, Cerri Paulo Sérgio, Marcantonio Elcio, Marcantonio Rosemary Adriana Chiérici
Araraquara Dental School-São Paulo University "Júlio de Mesquita Filho", Araraquara, São Paulo, Brazil.
Lasers Med Sci. 2015 Nov;30(8):2095-103. doi: 10.1007/s10103-015-1731-8. Epub 2015 Mar 27.
The purpose of this study was to evaluate the erbium, chromium:yttrium-scandiumgallium-garnet (Er,Cr:YSGG) laser irradiation in the treatment of periodontitis in rats exposed to cigarette smoke inhalation (CSI). Ligatures were placed in the maxillary second molars. After a 15-day period, the ligatures were removed and 180 animals were randomly divided into six groups: (1) CSRP group--CSI and manual scaling and root planing (SRP) treatment; (2) CL group--CSI and Er,Cr:YSGG laser irradiation; (3) CSRP + L group-CSI, SRP, and Er,Cr:YSGG irradiation; (4) SRP group-manual SRP; (5) L group--Er,Cr:YSGG irradiation; (6) SRP + L group--SRP and Er,Cr:YSGG irradiation. At 7, 15, and 30 days after treatments, animals were euthanized and histologic, histometric, immunohistochemistry, and real-time PCR analyses were performed. Histometrically, no differences were observed in the SRP, L, and SRP + L groups exposed to CSI. The CSRP group showed more bone formation at 30 days than at 15 days (p < 0.01) but less bone at 30 days than the CL group at 30 days (p < 0.05). Immunohistochemical staining was positive for osteoblasts, fibroblasts, and osteoclasts. Real-time PCR showed more (vascular endothelial growth factor) VEGF expression in the L (p < 0.05) and SRP + L (p < 0.01) groups at 30 days than at 15 days and less VEGF expression in the CSRP group at 30 days than at 15 days (p < 0.05). There was no difference in fibroblast growth factor (FGF) expression. The Er,Cr:YSGG laser irradiation promotes favorable conditions for tissue repair even in animals exposed to CSI, with similar results as those achieved from manual scaling and root planing.
钇钪镓石榴石（Er,Cr:YSGG）激光照射对吸入香烟烟雾（CSI）的大鼠牙周炎的治疗效果。在上颌第二磨牙放置结扎丝。15天后，移除结扎丝，将180只动物随机分为六组：（1）CSRP组——CSI并进行手工龈上洁治和根面平整（SRP）治疗；（2）CL组——CSI并接受Er,Cr:YSGG激光照射；（3）CSRP + L组——CSI、SRP和Er,Cr:YSGG照射；（4）SRP组——手工SRP；（5）L组——Er,Cr:YSGG照射；（6）SRP + L组——SRP和Er,Cr:YSGG照射。在治疗后7天、15天和30天，对动物实施安乐死，并进行组织学、组织计量学、免疫组织化学和实时聚合酶链反应分析。组织计量学分析显示，暴露于CSI的SRP组、L组和SRP + L组未观察到差异。CSRP组在30天时的骨形成比15天时更多（p < 0.01），但在30天时的骨量比CL组在30天时更少（p < 0.05）。免疫组织化学染色显示成骨细胞、成纤维细胞和破骨细胞呈阳性。实时聚合酶链反应显示，L组（p < 0.05）和SRP + L组（p < 0.01）在30天时的（血管内皮生长因子）VEGF表达比15天时更多，而CSRP组在30天时的VEGF表达比15天时更少（p < 0.05）。成纤维细胞生长因子（FGF）表达无差异。即使在暴露于CSI的动物中，Er,Cr:YSGG激光照射也能促进有利于组织修复的条件，其结果与手工龈上洁治和根面平整相似。
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