Araraquara Dental School, São Paulo State University Júlio de Mesquita Filho, Araraquara, São Paulo, Brazil.
Lasers Med Sci. 2012 Jan;27(1):95-102. doi: 10.1007/s10103-011-0920-3. Epub 2011 May 5.
The purpose of this study was to investigate the histological changes that occur in rat soft and hard tissues after Er,Cr:YSGG laser surgery. Each of 20 rats was submitted to four procedures which were randomly distributed to the right and left sides of the animal: procedure 1 dorsal incision with a scalpel; procedure 2 dorsal incision with a 2.0-W Er,Cr:YSGG laser; procedure 3 skull defect created with a diamond bur; procedure 4 skull defect created with a 3.0-W Er,Cr:YSGG laser. The animals were killed 3, 7, 15 and 30 days after surgery, and histological examinations were performed. The histometric analysis of the bone defects was evaluated using an unpaired t-test. Initially, the dorsum showed more histological signs of repair following procedure 1, although similar healing responses following procedures 1 and 2 were seen on day 30 after surgery. By day 30 the bone formation observed following procedure 4 was much more evident than following procedure 3. The unpaired t-test identified significant differences in bone formation on day 30 (p = 0.01), whereas a greater bone percentage was seen following procedure 4 than following procedure 3 (79.96 ± 10.30% and 58.23 ± 9.99%, respectively). Thus, histological repair of the Er,Cr:YSGG laser wounds was similar to that of the scalpel wounds. However, skull defects created with the Er,Cr:YSGG laser showed greater bone formation than defects created with the bur. Within the limitations of this study, we can conclude that the Er,Cr:YSGG laser is a promising surgical instrument in vivo, particularly for bone surgery.
YSGG 激光手术对大鼠软硬组织的组织学变化。将 20 只大鼠分为四组，每只大鼠的右侧和左侧随机接受以下四种处理之一：1 组为手术刀背部切口；2 组为 2.0W Er,Cr:YSGG 激光背部切口；3 组为钻石磨头造成颅骨缺损；4 组为 3.0W Er,Cr:YSGG 激光造成颅骨缺损。手术后 3、7、15 和 30 天处死动物，进行组织学检查。使用独立样本 t 检验对骨缺损的组织学分析进行评估。最初，第 1 组的背部显示出更多的组织修复迹象，尽管手术后第 30 天，第 1 组和第 2 组的愈合反应相似。到第 30 天，第 4 组观察到的骨形成明显多于第 3 组。独立样本 t 检验在第 30 天（p=0.01）发现骨形成有显著差异，而第 4 组的骨比例明显高于第 3 组（分别为 79.96±10.30%和 58.23±9.99%）。因此，Er,Cr:YSGG 激光伤口的组织学修复与手术刀伤口相似。然而，与磨头造成的颅骨缺损相比，Er,Cr:YSGG 激光造成的颅骨缺损具有更大的骨形成。在本研究的限制内，我们可以得出结论，Er,Cr:YSGG 激光是一种有前途的体内手术器械，特别是用于骨手术。
