Lee Jeung-Eun, Kim Jung-Min, Jang Hyun-Jun, Lim Se-Young, Choi Seon-Jeong, Lee Nan-Hee, Suh Pann-Ghill, Choi Ung-Kyu
School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan 689-798, Korea.
Division of Molecular and Life sciences, Pohang University of Science and Technology, Pohang, Korea.
Mol Cells. 2015 Apr;38(4):336-42. doi: 10.14348/molcells.2015.2238. Epub 2015 Mar 26.
Propyl gallate (PG) used as an additive in various foods has antioxidant and anti-inflammatory effects. Although the functional roles of PG in various cell types are well characterized, it is unknown whether PG has effect on stem cell differentiation. In this study, we demonstrated that PG could inhibit adipogenic differentiation in human adipose tissue-derived mesenchymal stem cells (hAMSCs) by decreasing the accumulation of intracellular lipid droplets. In addition, PG significantly reduced the expression of adipocyte-specific markers including peroxisome proliferator-activated receptor-γ (PPAR-γ), CCAAT enhancer binding protein-α (C/EBP-α), lipoprotein lipase (LPL), and adipocyte fatty acid-binding protein 2 (aP2). PG inhibited adipogenesis in hAMSCs through extracellular regulated kinase (ERK) pathway. Decreased adipogenesis following PG treatment was recovered in response to ERK blocking. Taken together, these results suggest a novel effect of PG on adipocyte differentiation in hAMSCs, supporting a negative role of ERK1/2 pathway in adipogenic differentiation.
没食子酸丙酯（PG）作为一种添加剂用于各类食品中，具有抗氧化和抗炎作用。尽管PG在各种细胞类型中的功能作用已得到充分表征，但尚不清楚PG是否对干细胞分化有影响。在本研究中，我们证明PG可通过减少细胞内脂滴的积累来抑制人脂肪组织来源的间充质干细胞（hAMSCs）的成脂分化。此外，PG显著降低了脂肪细胞特异性标志物的表达，包括过氧化物酶体增殖物激活受体-γ（PPAR-γ）、CCAAT增强子结合蛋白-α（C/EBP-α）、脂蛋白脂肪酶（LPL）和脂肪细胞脂肪酸结合蛋白2（aP2）。PG通过细胞外调节激酶（ERK）途径抑制hAMSCs的成脂作用。在ERK阻断后，PG处理后减少的成脂作用得以恢复。综上所述，这些结果表明PG对hAMSCs的脂肪细胞分化具有新的作用，支持ERK1/2途径在成脂分化中的负向作用。
